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Endogenous KLF4 Expression in Human Fetal Endothelial Cells Allows for Reprogramming to Pluripotency With Just OCT3/4 and SOX2—Brief Report
- Source :
- Arteriosclerosis, Thrombosis, and Vascular Biology. 30:1905-1907
- Publication Year :
- 2010
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2010.
-
Abstract
- Objective— The introduction of 4 transcription factors—c-MYC, OCT3/4, SOX2, and KLF4—can reprogram somatic cells back to pluripotency. However, some of the factors used are oncogenic, making therapeutic application unfeasible. Although the use of adult stem cells expressing high endogenous levels of some of these factors allows for reprogramming with fewer exogenous genes, such cells are rare and may have accumulated genetic mutations. Our goal was to reprogram human somatic cells without oncogenic factors. We found that high endogenous expression of KLF4 in human umbilical vein endothelial cells (HUVECs) allows for generation of induced pluripotent stem cells (iPSCs) with just 2 nononcogenic factors, OCT3/4 and SOX2 . Methods and Results— HUVECs were infected with lentivirus containing OCT4 and SOX2 for generation of iPSCs. These 2-factor HUVEC iPSCs were morphologically similar to embryonic stem cells, express endogenous pluripotency markers postreprogramming, and can differentiate toward lineages of all 3 germ layers both in vitro and in vivo. Conclusion— iPSCs can be generated from HUVECs with only 2 nononcogenic factors. The use of fetal cells for reprogramming without oncogenic factors may provide an efficient in vitro model for human iPSC research, as well as a novel source for possible therapeutic use.
- Subjects :
- Pluripotent Stem Cells
Cellular differentiation
Kruppel-Like Transcription Factors
Gene Expression
In Vitro Techniques
Biology
Transfection
Article
Kruppel-Like Factor 4
SOX2
Humans
Induced pluripotent stem cell
Cells, Cultured
Embryonic Stem Cells
SOXB1 Transcription Factors
Lentivirus
Endothelial Cells
Cell Differentiation
Cellular Reprogramming
Embryonic stem cell
Recombinant Proteins
Cell biology
KLF4
Immunology
Stem cell
Cardiology and Cardiovascular Medicine
Octamer Transcription Factor-3
Reprogramming
Adult stem cell
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....a1297e9b34914080492865bd1e855e4b