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Activation of alveolar macrophage TNF and MCP-1 expression in vivo by a synthetic peptide of C-reactive protein
- Source :
- Journal of Leukocyte Biology. 59:397-402
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- Administration of multilamellar vesicles (MLV) encapsulating a synthetic peptide (RS-83277) derived from human C-reactive protein (CRP) augments anti-tumor activity of murine alveolar macrophages and reduces established pulmonary metastases of experimental tumors. To explore mechanisms involved in these phenomena, we investigated cytokine and integrin (CD11b) expression of bronchoalveolar lavage (BAL)-derived alveolar macrophages in control (blank MLV) and RS-83277-MLV-treated C57B1 mice. Alveolar macrophage production of tumor necrosis factor α (TNF-α) and monocyte chemoattractant bioactivity increased at 48 h after treatment with RS-83277-MLV but not control MLV. Chemoattractant activity was neutralized by antibody to monocyte chemoattractant protein-1 (MCP-1), but not irrelevant immunoglobulin G (IgG). Changes were reflected by augmented TNF-α and MCP-1 mRNA levels in pulmonary tissue and enhanced CD11b expression on mononuclear leukocytes derived from total lung tissue, but not on BAL-derived alveolar macrophages. Results suggest that RS-83277-MLV treatment is associated with activation of alveolar macrophage TNF-α and MCP-1 production and up-regulation of adhesion molecules on pulmonary mononuclear leukocytes but not on alveolar macrophages.
- Subjects :
- Male
Chemokine
medicine.medical_treatment
Molecular Sequence Data
Immunology
Gene Expression
Biology
Immunoglobulin G
Mice
Macrophages, Alveolar
medicine
Animals
Immunologic Factors
Immunology and Allergy
Amino Acid Sequence
RNA, Messenger
Chemoattractant activity
Chemokine CCL2
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Monocyte
Cell Biology
Macrophage Activation
respiratory system
Molecular biology
Peptide Fragments
Mice, Inbred C57BL
C-Reactive Protein
Bronchoalveolar lavage
medicine.anatomical_structure
Cytokine
Alveolar macrophage
biology.protein
Cytokines
Intercellular Signaling Peptides and Proteins
Tumor necrosis factor alpha
Chemokines
Peptides
Bronchoalveolar Lavage Fluid
Subjects
Details
- ISSN :
- 19383673 and 07415400
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Journal of Leukocyte Biology
- Accession number :
- edsair.doi.dedup.....a12bbb57a69291888403fb9a35f6f3db