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Acridine Orange Excited by Low-Dose Radiation Has a Strong Cytocidal Effect on Mouse Osteosarcoma
- Source :
- Oncology. 62:85-93
- Publication Year :
- 2002
- Publisher :
- S. Karger AG, 2002.
-
Abstract
- The study was conducted to clarify the cytocidal effect of combination therapy consisting of administration of acridine orange (AO), which is a photosensitizer, and radiation therapy using in vitro and in vivo mouse osteosarcoma models. The results revealed that AO combined with low-dose X-ray irradiation of about 1–5 Gy had a strong cytocidal effect on the cultured mouse osteosarcoma cells regardless of their chemosensitivity, and that this combination therapy inhibited growth of the in vivo mouse osteosarcoma by induction of tumor necrosis. This effect was inhibited by L-histidine, but not by mannitol. These findings suggested that AO might be excited by X-rays and kill osteosarcoma cells through the release of singlet oxygen, which is toxic to living cells. This mechanism is similar to that of photodynamic therapy with AO.
- Subjects :
- Cancer Research
Lung Neoplasms
Time Factors
Combination therapy
Cell Survival
medicine.medical_treatment
Photodynamic therapy
Mice
chemistry.chemical_compound
In vivo
Tumor Cells, Cultured
medicine
Animals
Photosensitizer
Cell Size
Osteosarcoma
Chemistry
business.industry
X-Rays
Acridine orange
Dose-Response Relationship, Radiation
General Medicine
medicine.disease
Acridine Orange
In vitro
Survival Rate
Radiation therapy
Microscopy, Fluorescence
Photochemotherapy
Oncology
Cancer research
Nuclear medicine
business
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 14230232 and 00302414
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Oncology
- Accession number :
- edsair.doi.dedup.....a1346de9cb99c1cf33264d8fa9051248