Back to Search
Start Over
Study of the Role of the Tyrosine Kinase Receptor MerTK in the Development of Kidney Ischemia-Reperfusion Injury in RCS Rats
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩, International Journal of Molecular Sciences, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩, Volume 22, Issue 22, International Journal of Molecular Sciences, Vol 22, Iss 12103, p 12103 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Renal ischaemia reperfusion (I/R) triggers a cascade of events including oxidative stress, apoptotic body and microparticle (MP) formation as well as an acute inflammatory process that may contribute to organ failure. Macrophages are recruited to phagocytose cell debris and MPs. The tyrosine kinase receptor MerTK is a major player in the phagocytosis process. Experimental models of renal I/R events are of major importance for identifying I/R key players and for elaborating novel therapeutical approaches. A major aim of our study was to investigate possible involvement of MerTK in renal I/R. We performed our study on both natural mutant rats for MerTK (referred to as RCS) and on wild type rats referred to as WT. I/R was established by of bilateral clamping of the renal pedicles for 30′ followed by three days of reperfusion. Plasma samples were analysed for creatinine, aspartate aminotransferase (ASAT), lactate dehydrogenase (LDH), kidney injury molecule -1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) levels and for MPs. Kidney tissue damage and CD68-positive cell requirement were analysed by histochemistry. monocyte chemoattractant protein-1 (MCP-1), myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS), and histone 3A (H3A) levels in kidney tissue lysates were analysed by western blotting. The phagocytic activity of blood-isolated monocytes collected from RCS or WT towards annexin-V positive bodies derived from cultured renal cell was assessed by fluorescence-activated single cell sorting (FACS) and confocal microscopy analyses. The renal I/R model for RCS rat described for the first time here paves the way for further investigations of MerTK-dependent events in renal tissue injury and repair mechanisms.
- Subjects :
- Nitric Oxide Synthase Type II
030204 cardiovascular system & hematology
Kidney
Receptor tyrosine kinase
ischemia-reperfusion
chemistry.chemical_compound
0302 clinical medicine
Biology (General)
Spectroscopy
Chemokine CCL2
microparticles
0303 health sciences
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
biology
phagocytosis
General Medicine
Acute Kidney Injury
Apoptotic body
Computer Science Applications
Chemistry
medicine.anatomical_structure
Myeloperoxidase
Creatinine
Reperfusion Injury
RCS rats
medicine.medical_specialty
QH301-705.5
Nitric Oxide
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
MerTK
Lipocalin-2
Internal medicine
medicine
Animals
Humans
Aspartate Aminotransferases
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
030304 developmental biology
Peroxidase
Renal ischemia
L-Lactate Dehydrogenase
c-Mer Tyrosine Kinase
business.industry
Macrophages
Organic Chemistry
MERTK
medicine.disease
Rats
Endocrinology
chemistry
inflammation
biology.protein
business
Reperfusion injury
Cell Adhesion Molecules
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩, International Journal of Molecular Sciences, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩, Volume 22, Issue 22, International Journal of Molecular Sciences, Vol 22, Iss 12103, p 12103 (2021)
- Accession number :
- edsair.doi.dedup.....a19f686ea9beaf070d18f312cff0e2a8