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Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- We evaluated the dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells (CDCs), a mixed cell population grown from heart tissues. Adult C57BL/6 mice were exposed to 0, 10, 50 and 250 mGy γ 3-rays for 7 days and atrial tissues were collected for experiments 24 hours after last exposure. The number of CDCs was significantly decreased by daily exposure to over 250 mGy. Interestingly, daily exposure to over 50 mGy significantly decreased the c-kit expression and telomerase activity, increased 53BP1 foci in the nuclei of CDCs. However, CD90 expression and growth factors production in CDCs were not significantly changed even after daily exposure to 250 mGy. We further evaluated the reversibility of radiation-induced injury in CDCs at 1 week and 3 weeks after a single exposure to 3 Gy γ 3-rays. The number and growth factors production of CDCs were soon recovered at 1 week. However, the increased expression of CD90 were retained at 1 week, but recovered at 3 weeks. Moreover, the decreased expression of c-kit, impaired telomerase activity, and increased 53BP1 foci were poorly recovered even at 3 weeks. These data may help us to find the most sensitive and reliable bio-parameter(s) for evaluating radiation-induced injury in CDCs.<br />Scientific Reports, 7, 40959; 2017
- Subjects :
- 0301 basic medicine
Telomerase
Cell Survival
Cells
Population
Dose dependence
Enzyme-Linked Immunosorbent Assay
Radiation induced
Radiation Dosage
Article
Andrology
03 medical and health sciences
0302 clinical medicine
Animals
Medicine
CD90
Daily exposure
Radiation Injuries
education
education.field_of_study
Multidisciplinary
business.industry
Gene Expression Profiling
Myocardium
Heart
Mice, Inbred C57BL
030104 developmental biology
030220 oncology & carcinogenesis
Heart tissues
business
Explant culture
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....a1c0197aab24fd4be6b9dcc58edb73bb
- Full Text :
- https://doi.org/10.1038/srep40959