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Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
- Source :
- Micromachines, Vol 9, Iss 6, p 286 (2018), Micromachines, Volume 9, Issue 6
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Microfluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential operation in microfluidic systems, especially in investigating large-scale combination conditions. Here, we develop a nanoliter centrifugal liquid dispenser (NanoCLD) coupled with superhydrophobic microwell array chips for high-throughput cell-based assays in the nanoliter scale. The NanoCLD consists of a plastic stock block with an array of drilled through holes, a reagent microwell array chip (reagent chip), and an alignment bottom assembled together in a fixture. A simple centrifugation at 800 rpm can dispense ~160 nL reagents into microwells in 5 min. The dispensed reagents are then delivered to cells by sandwiching the reagent chip upside down with another microwell array chip (cell chip) on which cells are cultured. A gradient of doxorubicin is then dispensed to the cell chip using the NanoCLD for validating the feasibility of performing drug tests on our microchip platform. This novel nanoliter-volume liquid dispensing method is simple, easy to operate, and especially suitable for repeatedly dispensing many different reagents simultaneously to microwells.
- Subjects :
- 0301 basic medicine
Materials science
Mechanical Engineering
lcsh:Mechanical engineering and machinery
Microfluidics
microfluidics
Nanotechnology
Cell chip
Chip
microwell array
high-throughput screening
Article
03 medical and health sciences
nanoliter liquid dispensing
030104 developmental biology
Control and Systems Engineering
Reagent
lcsh:TJ1-1570
drug screening
Electrical and Electronic Engineering
superhydrophobicity
Subjects
Details
- Language :
- English
- Volume :
- 9
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Micromachines
- Accession number :
- edsair.doi.dedup.....a1c357b12b8ee781d670ab41705c20f6