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Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells from Patients with Crohn’s Disease
- Source :
- International Journal of Molecular Sciences, Volume 24, Issue 5, Pages: 4554
- Publication Year :
- 2023
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2023.
-
Abstract
- Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) which is the precursor of the photosensitizer protoporphyrin IX (PpIX) is an available treatment for several diseases. ALA-PDT induces the apoptosis and necrosis of target lesions. We have recently reported the effects of ALA-PDT on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). This study has investigated the ALA-PDT-mediated effects on PBMC subsets from patients with active Crohn’s disease (CD). No effects on lymphocyte survival after ALA-PDT were observed, although the survival of CD3−/CD19+ B-cells seemed slightly reduced in some samples. Interestingly, ALA-PDT clearly killed monocytes. The subcellular levels of cytokines and exosomes associated with inflammation were widely downregulated, which is consistent with our previous findings in PBMCs from healthy human subjects. These results suggest that ALA-PDT may be a potential treatment candidate for CD and other immune-mediated diseases.
- Subjects :
- Crohn’s disease
flow cytometry
Organic Chemistry
peripheral blood mononuclear cells (PBMCs)
autoimmune disease
General Medicine
Catalysis
Computer Science Applications
photodynamic therapy (PDT)
Inorganic Chemistry
5-aminolevulinic acid (ALA)
extracorporeal photopheresis (ECP)
cytokine
exosome
Physical and Theoretical Chemistry
protoporphyrin IX (PpIX)
Molecular Biology
Spectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a1cacc53ab6a81727bdba61b873732e4
- Full Text :
- https://doi.org/10.3390/ijms24054554