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Enhanced serodiagnostic potential of a fusion molecule consisting of Rv1793, Rv2628 and a truncated Rv2608 of Mycobacterium tuberculosis
- Source :
- PloS one, vol 16, iss 11, PLoS ONE, PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE, Vol 16, Iss 11, p e0258389 (2021)
- Publication Year :
- 2021
- Publisher :
- eScholarship, University of California, 2021.
-
Abstract
- Serodiagnosis of tuberculosis (TB) can be rapid, reliable and cost-effective if the issue of variable antibody responses of TB patients against different Mycobacterium tuberculosis (Mtb) antigens can be overcome by developing fusion proteins containing epitopes from multiple antigens of Mtb. In this study, Mtb antigens Rv1793, Rv2628, Rv2608 and a truncated variant produced by removing non-epitopic region from N-terminal of Rv2608 (tnRv2608), and the fusion protein Rv1793-Rv2628-tnRv2608 (TriFu64), were expressed in E. coli and purified. Plasma samples from TB patients characterized by sex, age and sputum/culture positivity, were used to compare the sensitivity of the single antigens with the fusion protein. Sensitivity of Rv1793, Rv2628 and Rv2608, was 27.8%, 39% and 36.3%, respectively. Truncation of Rv2608 increased sensitivity by approximately 35% in confirmed TB cases. Sensitivity of the fusion construct, TriFu64 increased to 66% with a specificity of 100%. Importantly, tnRv2608 was better able to detect sputum and culture negative patients, and this carried through to the fusion protein. We demonstrate that fusion of Mtb proteins ensures broad sensitivity across disease types, sex and age groups in a Pakistani population.
- Subjects :
- Bacterial Diseases
Male
B Cells
Physiology
Extensively Drug-Resistant Tuberculosis
Biochemistry
Epitope
White Blood Cells
Epitopes
Medical Conditions
Animal Cells
Immune Physiology
Medicine and Health Sciences
80 and over
Pakistan
Enzyme-Linked Immunoassays
Lung
Aged, 80 and over
Fusion
Multidisciplinary
Immune System Proteins
biology
Plasma samples
Bacterial
Pulmonary
Middle Aged
Antibodies, Bacterial
Body Fluids
Actinobacteria
Blood
Infectious Diseases
Medicine
Female
medicine.symptom
Anatomy
Cellular Types
Infection
Research Article
Biotechnology
Adult
Tuberculosis
Adolescent
General Science & Technology
Science
Immune Cells
Recombinant Fusion Proteins
Immunology
Research and Analysis Methods
Sensitivity and Specificity
Antibodies
Mycobacterium tuberculosis
Vaccine Related
Young Adult
Rare Diseases
Antigen
Bacterial Proteins
Biodefense
medicine
Escherichia coli
Humans
Serologic Tests
Antigens
Immunoassays
Antibody-Producing Cells
Tuberculosis, Pulmonary
Aged
Antigens, Bacterial
Blood Cells
Bacteria
Prevention
Organisms
Sputum
Biology and Life Sciences
Proteins
Cell Biology
Blood Serum
medicine.disease
biology.organism_classification
Tropical Diseases
Fusion protein
Virology
Mucus
Emerging Infectious Diseases
Good Health and Well Being
Immunologic Techniques
Immune Serum
Mycobacterium Tuberculosis
Follow-Up Studies
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PloS one, vol 16, iss 11, PLoS ONE, PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE, Vol 16, Iss 11, p e0258389 (2021)
- Accession number :
- edsair.doi.dedup.....a1d4a0aa02bb4422c37bc22ae8c59d4b