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PLOS ONE

PLOS ONE

Authors :
Chad C. Cheetham
Huan-Xin Chen
Fumiaki Yokoi
J. David Sweatt
Mai Tu Dang
Steven N. Roper
Susan Campbell
Yuqing Li
Animal and Poultry Sciences
Source :
PLoS ONE, PLoS ONE, Vol 10, Iss 3, p e0120916 (2015)
Publication Year :
2015
Publisher :
Public Library of Science, 2015.

Abstract

DYT1 dystonia is an inherited movement disorder caused by mutations in DYT1 (TOR1A), which codes for torsinA. Most of the patients have a trinucleotide deletion (ΔGAG) corresponding to a glutamic acid in the C-terminal region (torsinAΔE). Dyt1 ΔGAG heterozygous knock-in (KI) mice, which mimic ΔGAG mutation in the endogenous gene, exhibit motor deficits and deceased frequency of spontaneous excitatory post-synaptic currents (sEPSCs) and normal theta-burst-induced long-term potentiation (LTP) in the hippocampal CA1 region. Although Dyt1 KI mice show decreased hippocampal torsinA levels, it is not clear whether the decreased torsinA level itself affects the synaptic plasticity or torsinAΔE does it. To analyze the effect of partial torsinA loss on motor behaviors and synaptic transmission, Dyt1 heterozygous knock-out (KO) mice were examined as a model of a frame-shift DYT1 mutation in patients. Consistent with Dyt1 KI mice, Dyt1 heterozygous KO mice showed motor deficits in the beam-walking test. Dyt1 heterozygous KO mice showed decreased hippocampal torsinA levels lower than those in Dyt1 KI mice. Reduced sEPSCs and normal miniature excitatory post-synaptic currents (mEPSCs) were also observed in the acute hippocampal brain slices from Dyt1 heterozygous KO mice, suggesting that the partial loss of torsinA function in Dyt1 KI mice causes action potential-dependent neurotransmitter release deficits. On the other hand, Dyt1 heterozygous KO mice showed enhanced hippocampal LTP, normal inputoutput relations and paired pulse ratios in the extracellular field recordings. The results suggest that maintaining an appropriate torsinA level is important to sustain normal motor performance, synaptic transmission and plasticity. Developing therapeutics to restore a normal torsinA level may help to prevent and treat the symptoms in DYT1 dystonia. This work was supported by Tyler’s Hope for a Dystonia Cure, Inc., National Institutes of Health grants (NS37409, NS47466, NS47692, NS54246, NS57098, NS65273, NS72872, NS 74423, and NS82244), Howard Hughes Med-Grad Graduate Fellowship (CCC), and startup funds from the Lucille P. Markey Charitable Trust (UIUC) and Department of Neurology (UAB and UF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Details

Language :
English
ISSN :
19326203
Volume :
10
Issue :
3
Database :
OpenAIRE
Journal :
PLoS ONE
Accession number :
edsair.doi.dedup.....a1eba79d243d5bd7f057cbc457f5b62a