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Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle
- Source :
- Nucleic Acids Research
- Publication Year :
- 2015
-
Abstract
- Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated into myotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications.
- Subjects :
- Histones/*metabolism
Muscle Fibers, Skeletal/metabolism
Muscle, Skeletal/*embryology/metabolism
Muscle Fibers, Skeletal
Biology
Muscle Development
Methylation
Cell Line
Histones
Histone H3
Mice
Cell Lineage/genetics
Genetics
medicine
Animals
Cell Lineage
Muscle, Skeletal
Promoter Regions, Genetic
Regulation of gene expression
Muscle Development/*genetics
Myogenesis
Gene regulation, Chromatin and Epigenetics
Skeletal muscle
Gene Expression Regulation, Developmental
Molecular biology
Chromatin
medicine.anatomical_structure
Histone
biology.protein
H3K4me3
C2C12
Subjects
Details
- Language :
- English
- ISSN :
- 03051048
- Volume :
- 43
- Issue :
- No. 2
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....a1f511865babc7d3c1a5f0e6f5b698a3