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Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Authors :
Aleksandr Khilov
Ekaterina A. Sergeeva
Mikhail Yu. Kirillin
Natalia Orlinskaya
Maria Shakhova
Alina Meller
D. A. Kurakina
Ilya V. Turchin
Source :
Journal of Biomedical Optics
Publication Year :
2019
Publisher :
SPIE-Intl Soc Optical Eng, 2019.

Abstract

Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. We provide a comparative analysis of different PDT regimes performed with blue and red lights and their combination, with doses varying from 50 to 150 J/cm2. The study was performed on the intact skin of a rabbit ear inner surface, with the use of chlorin e6 as a PS. PDT procedure protocol included monitoring of the treated site with fluorescence imaging technique to evaluate PS accumulation and photobleaching, as well as with optical coherence tomography (OCT) to register morphological and functional responses of the tissue. Optical diagnostic observations were compared with the results of histopathology examination. We demonstrated that PDT procedures with the considered regimes induce weaker organism reaction manifested by edema in normal tissue as compared to irradiation-only exposures with the same light doses. The light doses delivered with red light induce weaker tissue reaction as compared to the same doses delivered with blue light only or with a combination of red and blue lights in equal parts. Results of in-vivo OCT monitoring of tissue reaction are in agreement with the results of histopathology study.

Details

ISSN :
10833668
Volume :
25
Database :
OpenAIRE
Journal :
Journal of Biomedical Optics
Accession number :
edsair.doi.dedup.....a1fea3e8c5833643a5182094fbeb0c22
Full Text :
https://doi.org/10.1117/1.jbo.25.6.063804