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Oral butyrate reduces oxidative stress in atherosclerotic lesion sites by a mechanism involving NADPH oxidase down-regulation in endothelial cells
- Source :
- The Journal of nutritional biochemistry. 34
- Publication Year :
- 2015
-
Abstract
- Butyrate is a 4-carbon fatty acid that has antiinflammatory and antioxidative properties. It has been demonstrated that butyrate is able to reduce atherosclerotic development in animal models by reducing inflammatory factors. However, the contribution of its antioxidative effects of butyrate on atherogenesis has not yet been studied. We investigated the influence of butyrate on oxidative status, reactive oxygen species (ROS) release and oxidative enzymes (NADPH oxidase and iNOS) in atherosclerotic lesions of ApoE(-/-) mice and in oxLDL-stimulated peritoneal macrophages and endothelial cells (EA.hy926). The lesion area in aorta was reduced while in the aortic valve, although lesion area was unaltered, superoxide production and protein nitrosylation were reduced in butyrate-supplemented mice. Peritoneal macrophages from the butyrate group presented a lower free radical release after zymosan stimulus. When endothelial cells were pretreated with butyrate before oxLDL stimulus, the CCL-2 and superoxide ion productions and NADPH oxidase subunit p22phox were reduced. In macrophage cultures, in addition to a reduction in ROS release, nitric oxide and iNOS expression were down-regulated. The data suggest that one mechanism related to the effect of butyrate on atherosclerotic development is the reduction of oxidative stress in the lesion site. The reduction of oxidative stress related to NADPH oxidase and iNOS expression levels associated to butyrate supplementation attenuates endothelium dysfunction and macrophage migration and activation in the lesion site.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Nitric Oxide Synthase Type II
Butyrate
Biology
medicine.disease_cause
Biochemistry
Antioxidants
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
Mice, Knockout
Reactive oxygen species
Nutrition and Dietetics
NADPH oxidase
Protein nitrosylation
Superoxide
NADPH Oxidases
Macrophage Activation
Atherosclerosis
Lipoproteins, LDL
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Dietary Supplements
biology.protein
Macrophages, Peritoneal
Butyric Acid
P22phox
Endothelium, Vascular
Enzyme Repression
Reactive Oxygen Species
Oxidative stress
Biomarkers
Subjects
Details
- ISSN :
- 18734847
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The Journal of nutritional biochemistry
- Accession number :
- edsair.doi.dedup.....a22dcac75c3e8cfe3338f76ea8a0b6d9