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Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter
- Source :
- Proceedings of the National Academy of Sciences. 85:723-727
- Publication Year :
- 1988
- Publisher :
- Proceedings of the National Academy of Sciences, 1988.
-
Abstract
- A sequence centered 166 nucleotides upstream of the mouse H-2Kb class I major histocompatibility gene binds a nuclear factor, H2TF1, found in many cell types. Previous studies have shown that binding of H2TF1 to this sequence stimulates class I gene expression. Furthermore, this factor binds a similar sequence in the 72-base-pair repeat enhancer element of simian virus 40. We show here that NF-kappa B, an inducible B-cell-specific factor that binds the kappa immunoglobulin light chain gene enhancer, also binds the H2TF1 regulatory sequence. Methylation-interference experiments demonstrate that NF-kappa B closely interacts with six of the eight symmetrically positioned guanines that contact H2TF1. These experiments suggest that NF-kappa B may play a role in class I major histocompatibility gene expression and that H2TF1 and NF-kappa B may be related DNA-binding proteins.
- Subjects :
- Binding Sites
Guanine
Multidisciplinary
GATA6
biology
H-2 Antigens
Genes, MHC Class I
Nuclear Proteins
Major histocompatibility complex
Molecular biology
DNA-Binding Proteins
Mice
Upstream activating sequence
Enhancer Elements, Genetic
Regulatory sequence
MHC class I
biology.protein
Animals
Promoter Regions, Genetic
Enhancer
Gene
Transcription factor
Transcription Factors
Research Article
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....a23bbbc78748f1cf7908d73b4e0b480a