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A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor

Authors :
Shun Sawatsubashi
Ryoji Fujiki
Takashi Ito
Takuya Murata
Ken-ichi Takeyama
Saya Ito
Jinseon Lim
Shigeaki Kato
Takashi Ueda
Yue Zhao
Shuhei Kimura
Sally Fujiyama
Daiki Umetsu
Masahiko Tanabe
Eriko Suzuki
Publication Year :
2010
Publisher :
Cold Spring Harbor Laboratory Press, 2010.

Abstract

Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary gland, Drosophila DEK (dDEK) was genetically identified as a coactivator of EcR in both insect cells and intact flies. Biochemical purification and characterization of the complexes containing fly and human DEKs revealed that DEKs serve as histone chaperones via phosphorylation by forming complexes with casein kinase 2. Consistent with the preferential association of the DEK complex with histones enriched in active epigenetic marks, dDEK facilitated H3.3 assembly during puff formation. In some human myeloid leukemia patients, DEK was fused to CAN by chromosomal translocation. This mutation significantly reduced formation of the DEK complex, which is required for histone chaperone activity. Thus, the present study suggests that at least one histone chaperone can be categorized as a type of transcriptional coactivator for nuclear receptors.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....a25a2467d5564b1eb6cb68f27d5a8e7f