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Cytohesins/ARNO: the function in colorectal cancer cells
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 3, p e90997 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) are critical regulators of cell differentiation, survival, proliferation, and migration in cancers. This study found that ARNO (cytohesin-2), an activator of the EGF and IGF-I pathways, was more highly expressed in colorectal cancer tissue than in benign adjacent colorectal tissue. When ARNO-siRNA or the chemical inhibitor SecinH3 blocked ARNO, the downstream of the EGF and IGF-I pathways decreased in colorectal cell lines HT29 and HCT116. This blocking also weakened cell proliferation, invasion, and migration in vitro. Furthermore, EGF receptor (EGFR)-dependent colorectal tumor xenografts in nude mouse exerted anti-proliferative and growth suppression effects by injecting secineH3. These data suggested that inhibiting cytohesins or ARNO as cytoplasmic activators of EGFR and IGF-I in colorectal cancer resulted in anti-proliferation, reduced invasion, decreased migration, and suppressed growth in vivo and in vitro. Therefore, cytohesins or ARNO may be a potential therapy target for some colorectal cancer.
- Subjects :
- Male
Colorectal cancer
Cellular differentiation
medicine.medical_treatment
Cancer Treatment
lcsh:Medicine
Gene Expression
Receptor, IGF Type 1
Mice
Nude mouse
Epidermal growth factor
Cell Movement
Molecular Cell Biology
Gastrointestinal Cancers
lcsh:Science
Multidisciplinary
GTPase-Activating Proteins
Signaling in Selected Disciplines
Tumor Burden
ErbB Receptors
Cell Motility
Oncology
Gene Knockdown Techniques
Heterografts
Medicine
Signal transduction
Colorectal Neoplasms
Research Article
Signal Transduction
Biophysics
Cytokine Therapy
Gastroenterology and Hepatology
Biology
Cell Growth
Cell Line, Tumor
Gastrointestinal Tumors
medicine
Animals
Humans
Cell Proliferation
Oncogenic Signaling
Cell growth
Growth factor
lcsh:R
Correction
Cancers and Neoplasms
Triazoles
medicine.disease
biology.organism_classification
Molecular biology
Xenograft Model Antitumor Assays
Disease Models, Animal
Cell culture
Cancer research
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a26a168d6ed547cac7301ce25cc488ea