MOESM5 of Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering

Additional file 5: Figure S5. Verification of quadruple-locus deletion of cre-1, res-1, gh1-1 and alp-1 in selected transformants. (A) Schematic of homologous recombination (HR) of target genes mediated by Cas9, sgRNA and donor DNA. (B) PCR analysis of quadruple-gene deletion of cre-1, res-1, gh1-1 and alp-1 in selected transformants with paired primers (cre1/res1/gh1-1/alp1-out-F and cre1/res1/gh1-1/alp1-in-R). The expected length of disrupted transformants was 1.9Â kb, while that of the host strain M2 (rightmost lane) was 1.0Â kb.