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Mice heterozygous for the mld mutation have intermediate levels of myelin basic protein mRNA and its translation products
Mice heterozygous for the mld mutation have intermediate levels of myelin basic protein mRNA and its translation products
- Source :
- Brain research. 387(2)
- Publication Year :
- 1986
-
Abstract
- Myelin-deficiency (mld) is an autosomal recessive mutation in mice exhibiting a severe deficit in the synthesis of myelin basic protein (MBP). In order to understand the mechanisms involved in the regulation of MBP synthesis in the mld mutation, we examined the amount of MBP and MBP-specific mRNA in control, heterozygous and homozygous mld brains. In vitro translation of poly(A)+ RNA in a cell-free system, in situ hybridization, and filter hybridization with a radiolabelled probe pMBP-1 after dot or Northern blotting were used in this study. The levels of MBP and MBP-specific mRNA were very low but detectable in mld homozygotes, and intermediate in heterozygotes. MBP specific mRNA from mutants, and its translation products, were of normal size. These results show that the mld mutation is expressed co-dominantly in heterozygotes and affects a cis-acting regulatory element controlling the MBP gene.
- Subjects :
- Mutant
In situ hybridization
In Vitro Techniques
medicine.disease_cause
complex mixtures
Cellular and Molecular Neuroscience
Mice
Mice, Neurologic Mutants
Gene expression
medicine
Animals
Chemical Precipitation
Northern blot
RNA, Messenger
Molecular Biology
Gene
Messenger RNA
Mutation
biology
Brain
Nucleic Acid Hybridization
Myelin Basic Protein
Molecular biology
Myelin basic protein
Protein Biosynthesis
biology.protein
Immunologic Techniques
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 387
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Brain research
- Accession number :
- edsair.doi.dedup.....a2c2aa10c76de377e72063b6bd2ef4aa