Back to Search
Start Over
A highly sensitive and selective off-on fluorescent chemosensor for hydrazine based on coumarin β-diketone
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 188
- Publication Year :
- 2017
-
Abstract
- A coumarin-based sensor C1, namely 3-acetoacetylcoumarin was designed, synthesized and applied for hydrazine detection. Hydrazinolysis of the chemosensor gives a fluorescent coumarin-pyrazole product C1-N2H4 [3-(3-methyl-1H-pyrazol-5-yl)coumarin], and thus resulting in a prominent fluorescence off-on response toward hydrazine under physiological conditions. The probe is highly selective toward hydrazine over cations, anions and other biologically/environmentally abundant analytes. The detection limit of the probe is 3.2ppb. The sensing mechanism was supported by 1H NMR, IR, MS and DFT calculation. The application of the fluorescent probe in monitoring intracellular hydrazine in glioma cell line U251 was also demonstrated.
- Subjects :
- Analyte
Spectrophotometry, Infrared
Hydrazine
Molecular Conformation
02 engineering and technology
Environment
010402 general chemistry
Photochemistry
Crystallography, X-Ray
01 natural sciences
Mass Spectrometry
Analytical Chemistry
β diketone
chemistry.chemical_compound
Coumarins
Cell Line, Tumor
Humans
Instrumentation
Spectroscopy
Fluorescent Dyes
Detection limit
Chemistry
021001 nanoscience & nanotechnology
Coumarin
Fluorescence
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Highly sensitive
Kinetics
Hydrazines
Spectrometry, Fluorescence
Proton NMR
0210 nano-technology
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 188
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....a2eab41cfd69ccc9b51b152697c703ab