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5-Episinuleptolide Decreases the Expression of the Extracellular Matrix in Early Biofilm Formation of Multi-Drug Resistant Acinetobacter baumannii
- Source :
- Marine Drugs; Volume 14; Issue 8; Pages: 143, Marine Drugs, Vol 14, Iss 8, p 143 (2016), Marine Drugs
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Nosocomial infections and increasing multi-drug resistance caused by Acinetobacter baumannii have been recognized as emerging problems worldwide. Moreover, A. baumannii is able to colonize various abiotic materials and medical devices, making it difficult to eradicate and leading to ventilator-associated pneumonia, and bacteremia. Development of novel molecules that inhibit bacterial biofilm formation may be an alternative prophylactic option for the treatment of biofilm-associated A. baumannii infections. Marine environments, which are unlike their terrestrial counterparts, harbor an abundant biodiversity of marine organisms that produce novel bioactive natural products with pharmaceutical potential. In this study, we identified 5-episinuleptolide, which was isolated from Sinularia leptoclados, as an inhibitor of biofilm formation in ATCC 19606 and three multi-drug resistant A. baumannii strains. In addition, the anti-biofilm activities of 5-episinuleptolide were observed for Gram-negative bacteria but not for Gram-positive bacteria, indicating that the inhibition mechanism of 5-episinuleptolide is effective against only Gram-negative bacteria. The mechanism of biofilm inhibition was demonstrated to correlate to decreased gene expression from the pgaABCD locus, which encodes the extracellular polysaccharide poly-β-(1,6)-N-acetylglucosamine (PNAG). Scanning electron microscopy (SEM) indicated that extracellular matrix of the biofilm was dramatically decreased by treatment with 5-episinuleptolide. Our study showed potentially synergistic activity of combination therapy with 5-episinuleptolide and levofloxacin against biofilm formation and biofilm cells. These data indicate that inhibition of biofilm formation via 5-episinuleptolide may represent another prophylactic option for solving the persistent problem of biofilm-associated A. baumannii infections.
- Subjects :
- Acinetobacter baumannii
0301 basic medicine
beta-Glucans
030106 microbiology
Pharmaceutical Science
multi-drug resistant A. baumannii
Levofloxacin
Article
biofilm
Microbiology
Extracellular matrix
03 medical and health sciences
Drug Resistance, Multiple, Bacterial
Drug Discovery
Gene expression
medicine
Animals
Humans
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Biological Products
Cross Infection
biology
5-Episinuleptolide
Biofilm
Drug Synergism
biochemical phenomena, metabolism, and nutrition
5-episinuleptolide
Anthozoa
biology.organism_classification
medicine.disease
Anti-Bacterial Agents
Extracellular Matrix
lcsh:Biology (General)
Genes, Bacterial
Biofilms
Bacteremia
Microscopy, Electron, Scanning
Equipment Contamination
Diterpenes
Bacteria
Acinetobacter Infections
medicine.drug
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....a30851ab5acdf7d5a9c392fe84f0dd7a