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Trichomonas vaginalis Detection in Female Specimens with cobas® TV/MG for use on the cobas® 6800/8800 Systems
- Source :
- European Journal of Microbiology & Immunology, European Journal of Microbiology & Immunology, Vol 9, Iss 2, Pp 42-45 (2019)
- Publication Year :
- 2019
- Publisher :
- Akadémiai Kiadó, 2019.
-
Abstract
- Trichomoniasis, a common curable sexually transmitted infection caused by the protozoan Trichomonas vaginalis (TV), is usually asymptomatic. However, symptomatic women may experience vaginal discharge and/or vulvar irritation. This study evaluated cobas® TV/ Mycoplasma genitalium (MG) (Conformité Européene marking for in vitro diagnostic medical devices [CE-IVD]) against other nucleic acid amplification tests (NAATs) for detecting TV in female urogenital specimens. Matched de-identified specimens from 412 females were collected. cobas® TV/MG results were compared against a composite reference (CR) of 3 different NAATs for TV (Aptima TV, modified S-DiaMGTV™, and a laboratory-developed test). The overall TV prevalence rate was 6.2%, based on cobas® TV/MG results. Relative to the CR, cobas® TV/MG sensitivity/specificity for the specimen types were endocervical swabs (ES) 100%/99.2%, vaginal swabs (VS) 100%/99.7%, urine (U) 100%/99.7%, and cervical specimens in PreservCyt® solution (PC) 100%/99.5%. There was no significant statistical difference between clinician-collected and self-collected VS (p = 0.28). Correlation of cobas® TV/MG vs. Aptima TV demonstrated the following positive, negative, and overall percent agreements, respectively: ES 69.0%, 98.7%, and 96.6%; VS 88.9%, 99.5%, and 98.8%; U 100%, 100%, and 100%; and PC 95.5%, 99.0%, and 98.8%. Detection of TV with cobas® TV/MG for use on the cobas® 6800/8800 systems demonstrated excellent performance in female urogenital specimens (overall sensitivity/specificity of 100%≥99.2%).
- Subjects :
- 0301 basic medicine
Trichomoniasis
business.industry
030106 microbiology
Usually asymptomatic
lcsh:QR1-502
Trichomonas vaginalis detection
medicine.disease_cause
medicine.disease
bacterial infections and mycoses
Virology
lcsh:Microbiology
Original Research Paper
molecular diagnostics
03 medical and health sciences
030104 developmental biology
parasitic diseases
medicine
Trichomonas vaginalis
sexually transmitted infection
business
cobas®
Subjects
Details
- Language :
- English
- ISSN :
- 20628633 and 2062509X
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- European Journal of Microbiology & Immunology
- Accession number :
- edsair.doi.dedup.....a30f22cf0b4c603717c325fdc6b601bf