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Single nucleotide polymorphism genotyping using locked nucleic acid (LNA)
- Source :
- Expert review of molecular diagnostics. 3(1)
- Publication Year :
- 2003
-
Abstract
- Locked nucleic acid (LNA) is a new class of bicyclic high affinity DNA analogs. LNA-containing oligonucleotides confer significantly increased affinity against their complementary DNA targets, increased mismatch discrimination (delta Tm) and allow full control of the melting point of the hybridization reaction. LNA chemistry is completely compatible with the traditional DNA phosphoramidite chemistry and therefore LNA-DNA mixmer oligonucleotides can be designed with complete freedom for optimal performance. These properties render LNA oligonucleotides very well suited for SNP genotyping and have enabled several approaches for enzyme-independent SNP genotyping based on allele-specific hybridization. In addition, allele-specific PCR assays relying on enzymatically-enhanced discrimination can be improved using LNA-modified oligonucleotides. The use of LNA transforms enzyme-independent genotyping approaches into experimentally simple, robust and cost-effective assays, which are highly suited for genotyping in clinical and industrial settings.
- Subjects :
- Genotype
Base Pair Mismatch
Molecular Sequence Data
Computational biology
Biology
Molecular Inversion Probe
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Pathology and Forensic Medicine
chemistry.chemical_compound
Nucleic acid thermodynamics
Genetics
Animals
Humans
Locked nucleic acid
Molecular Biology
Genotyping
Alleles
Oligonucleotide Array Sequence Analysis
Base Sequence
Oligonucleotide
Temperature
Nucleic Acid Hybridization
SNP genotyping
chemistry
Molecular Medicine
Oligonucleotide Probes
DNA
Subjects
Details
- ISSN :
- 14737159
- Volume :
- 3
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Expert review of molecular diagnostics
- Accession number :
- edsair.doi.dedup.....a331e163fb8ae6eeb9cbea5b2df59fa5