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Molecular Elucidation of a Urate Oxidase from Deinococcus radiodurans for Hyperuricemia and Gout Therapy
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 5611, p 5611 (2021), Volume 22, Issue 11
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Urate oxidase initiates the uric acid degradation pathways and is extensively used for protein drug development for gout therapy and serum uric acid diagnosis. We first present the biochemical and structural elucidation of a urate oxidase from the extremophile microorganism Deinococcus radiodurans (DrUox). From enzyme characterization, DrUox showed optimal catalytic ability at 30 °C and pH 9.0 with high stability under physiological conditions. Only the Mg2+ ion moderately elevated its activity, which indicates the characteristic of the cofactor-free urate oxidase family. Of note, DrUox is thermostable in mesophilic conditions. It retains almost 100% activity when incubated at 25 °C and 37 °C for 24 h. In this study, we characterized a thermostable urate oxidase, DrUox with high catalytic efficiency and thermal stability, which strengthens its potential for medical applications.
- Subjects :
- 0301 basic medicine
Gout
Urate Oxidase
QH301-705.5
Hyperuricemia
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Deinococcus radiodurans
medicine
Extremophile
Animals
Humans
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
QD1-999
Spectroscopy
chemistry.chemical_classification
030102 biochemistry & molecular biology
biology
Chemistry
gout therapy
Organic Chemistry
Urate oxidase
General Medicine
biology.organism_classification
medicine.disease
Recombinant Proteins
Computer Science Applications
030104 developmental biology
Enzyme
Biochemistry
Uric acid
uricase
Deinococcus
Mesophile
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a34de6705fd893c8da3a353141f1d3e2