Additional file 1 of 20-Hydroxyecdysone counteracts insulin to promote programmed cell death by modifying phosphoglycerate kinase 1

Additional file 1: Figure S1. The profiles of glucose and lactate concentrations in the hemolymph and PGK1 mRNA in the fat body. Figure S2. Phylogenetic tree and protein sequence alignment of PGK1 from H. armigera and other species based on amino acid sequence. Figure S3. PGK1 is phosphorylated at Tyr194. Figure S4. The interference efficiencies of the genes were detected separately by qRT-PCR. Figure S5. High 20E titer induces PGK1 acetylation at K386. Figure S6. Fat body autophagy and apoptosis during the feeding stage and wandering stage. Table S1. The PCR primer sequences used in this paper.