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Overexpression of Muscadinia rotundifolia CBF2 gene enhances biotic and abiotic stress tolerance in Arabidopsis
- Source :
- Protoplasma. 254(1)
- Publication Year :
- 2015
-
Abstract
- C-repeat-binding factor dehydration-responsive element-binding factor 1C (CBF2/DREB1C) gene encodes a small family of transcriptional activator that has been described as playing an important role in freezing tolerance and cold acclimation of plants. We here report that CBF2 gene also plays an important role in the early response to the pathogen infection of grapevine downy mildew disease. The expression level of CBF2 increased dramatically and reached a peak at 7 h after infection in immune grapevine Muscadinia rotundifolia 'Noble', which was much faster than moderate resistant Vitis amurensis 'PI1288' and susceptible Vitis vinifera 'Cabernet Sauvignon'. Muscadinia rotundifolia MrCBF2 exhibited amino acid domains characteristic of Vitis CBF2 proteins with unique features including rich serine repeats and slight differences in NLS, DSAWRL, and AP2 domains. The MrCBF2 gene was introduced to Arabidopsis 'COL0' which are susceptible to downy mildew pathogen. The transgenic lines showed an increased resistance to downy mildew disease and more accumulation of SA as well as higher expression of pathogenesis-related (PR) genes (AtPR1, AtPR4, and AtPR5) as a consequence of MrCBF2 overexpression. Besides, constitutive expression of MrCBF2 enhanced phytohormone abscisic acid (ABA)-independent drought tolerance of transgenic plants. Freezing tolerance of transgenic lines was also enhanced accompanied with an increase in the expression of the cold-regulated genes AtCOR, AtCOR15A, AtKIN1, AtRD29A, and AtSuSy. In addition, the development of MrCBF2-overexpressing plants was seen to be altered and resulted in growth retardation, dwarfism, late flowering, and prone rosette leaves, which may be because of an increase in the gene expression of partial DELLA proteins and DDF1.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Arabidopsis
Plant Science
Flowers
Plant disease resistance
Genes, Plant
Real-Time Polymerase Chain Reaction
01 natural sciences
Plant Epidermis
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Stress, Physiological
Botany
Gene expression
Onions
Cold acclimation
Vitis
wine.grape_variety
Amino Acid Sequence
Abscisic acid
Gene
Disease Resistance
Plant Diseases
Plant Proteins
Genetics
Cell Nucleus
Peronospora
biology
food and beverages
Cell Biology
General Medicine
biology.organism_classification
Plants, Genetically Modified
Adaptation, Physiological
Droughts
Cold Temperature
030104 developmental biology
chemistry
wine
Downy mildew
Sequence Alignment
Vitis amurensis
010606 plant biology & botany
Subcellular Fractions
Subjects
Details
- ISSN :
- 16156102
- Volume :
- 254
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....a369bba565618ee4797e3c319008f4c4