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The interaction between serum amyloid A and Tollālike receptor 2 pathway regulates inflammatory cytokine secretion in human gingival fibroblasts
- Source :
- Journal of Periodontology. 91:129-137
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- BACKGROUND Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis METHODS: SAA mRNA and protein expression were detected in healthy and inflammatory gingival tissues using real-time polymerase chain reaction (PCR) and immunohistochemistry. Human recombinant SAA (Apo-SAA), Pam3CSK4 (a Toll-like receptor (TLR) 2 ligand), siRNA-SAA, or TLR2 neutralizing antibody was applied to treat human gingival fibroblasts, respectively, or combined. SAA, TLRs, and inflammatory cytokines interleukin (IL)-6 and IL-8 were analyzed by real-time PCR, western blotting, or enzyme-linked immunosorbent assay. RESULTS SAA expression increased in human inflammatory gingival tissues from patients with periodontitis (P
- Subjects :
- 0301 basic medicine
animal diseases
Gingiva
Inflammation
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
hemic and lymphatic diseases
medicine
Humans
Serum amyloid A
Cells, Cultured
Periodontitis
Serum Amyloid A Protein
Toll-like receptor
business.industry
Interleukin
030206 dentistry
Fibroblasts
medicine.disease
Toll-Like Receptor 2
stomatognathic diseases
TLR2
030104 developmental biology
Immunology
Cytokines
Periodontics
Cytokine secretion
medicine.symptom
business
Subjects
Details
- ISSN :
- 19433670 and 00223492
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontology
- Accession number :
- edsair.doi.dedup.....a3800982d85c869d018ed169ab98a3d3