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Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study
- Source :
- Metabolites, Vol 11, Iss 518, p 518 (2021), Metabolites, Volume 11, Issue 8
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. A new imaging technique—named dynamic nuclear polarization (DNP) carbon-13 magnetic resonance imaging (MRI)—probes the glycolytic flux in a real-time, dynamic manner. The [1-13C]pyruvate is transported by the monocarboxylate transporter (MCT) into cells and converted into [1-13C]lactate by lactate dehydrogenase (LDH). To capture the early glycolytic alterations in the irradiated cancer and immune cells, we designed a preliminary DNP 13C-MRI study by using hyperpolarized [1-13C]pyruvate to study human FaDu squamous carcinoma cells, HMC3 microglial cells, and THP-1 monocytes before and after irradiation. The pyruvate-to-lactate conversion rate (kPL [Pyr.]) calculated by kinetic modeling was used to evaluate the metabolic alterations. Western blotting was performed to assess the expressions of LDHA, LDHB, MCT1, and MCT4 proteins. Following irradiation, the pyruvate-to-lactate conversion rates on DNP 13C-MRI were significantly decreased in the FaDu and the HMC3 cells but increased in the THP-1 cells. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. In conclusion, DNP 13C-MRI non-invasively captured the different glycolytic alterations among cancer and immune systems in response to irradiation, implying its potential for clinical use in the future.
- Subjects :
- Endocrinology, Diabetes and Metabolism
cancer metabolism
Microbiology
Biochemistry
Article
dynamic nuclear polarization
chemistry.chemical_compound
Immune system
Western blot
Lactate dehydrogenase
medicine
magnetic resonance imaging
Glycolysis
Molecular Biology
Monocarboxylate transporter
biology
medicine.diagnostic_test
Chemistry
glycolysis
QR1-502
Squamous carcinoma
radiation
Blot
immune system
biology.protein
Cancer research
Flux (metabolism)
Subjects
Details
- ISSN :
- 22181989
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Metabolites
- Accession number :
- edsair.doi.dedup.....a39fba11b1bbfed542f6120dca10bf15