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Role of peroxiredoxin 2 in H2O2-induced oxidative stress of primary Leydig cells
- Source :
- Molecular Medicine Reports. 13:4807-4813
- Publication Year :
- 2016
- Publisher :
- Spandidos Publications, 2016.
-
Abstract
- Late‑onset hypogonadism is defined as a condition caused by a decline in the levels of testosterone with aging. One of the major factors contributing to the low levels of testosterone is the accumulation of reactive oxygen species (ROS) in Leydig cells during the ageing process. Peroxiredoxin 2 (Prdx2), a member of the peroxiredoxin family, is an antioxidant protein, the predominant function of which is to neutralize ROS. However, its role in Leydig cells remains to be elucidated. In the present study, primary Leydig cells were exposed to low concentrations of hydrogen peroxide (H2O2) to induce oxidative stress. Cell apoptosis was measured using an Annexin V fluorescein isothiocyanate/propidium iodide apoptosis detection kit and flow cytometry. The level of testosterone was determined by radioimmunoassay, and the mRNA and protein expression levels of Prdx2 were detected by reverse transcription‑polymerase chain reaction and western blotting, respectively. The results revealed a significant increase in cell apoptosis and decrease in testosterone production. In addition, the expression of Prdx2 was decreased by H2O2 in a dose‑ and time‑dependent manner, and this decrease may have been caused by the induction of its molecular structure transformation due to H2O2 elimination. The above findings indicated that Prdx2 may prevent H2O2 accumulation in Leydig cells, and may be important in oxidative stress‑induced apoptosis and decreased testosterone production.
- Subjects :
- Male
0301 basic medicine
Cancer Research
medicine.medical_specialty
Cell Survival
Gene Expression
Apoptosis
Peroxiredoxin 2
Biology
medicine.disease_cause
Biochemistry
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Genetics
medicine
Animals
Testosterone
RNA, Messenger
Propidium iodide
Fluorescein isothiocyanate
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
Reactive oxygen species
Leydig Cells
Hydrogen Peroxide
Peroxiredoxins
Oxidative Stress
030104 developmental biology
Endocrinology
Oncology
chemistry
030220 oncology & carcinogenesis
Molecular Medicine
Lipid Peroxidation
Reactive Oxygen Species
Peroxiredoxin
Oxidative stress
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....a3a86a4de384f0b47b1ed0ef5baa352b