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Specific removal of anti-red cell blood group activity from anti-HLA lymphocytotoxic sera and purification of anti-A and anti-B antibodies with lymphocytotoxic activity
- Source :
- Tissue Antigens. 26:147-152
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Lymphocytotoxic antibodies to the red cell blood groups A and B were purified from human sera by elution from synthetic immunoadsorbents (Synsorbs from Chembiomed). The lymphocytotoxic activity of the eluted antibodies was better preserved when elution was carried out at pH 11, whereas it was less stable when elution was performed at pH 3. In addition, complete removal of anti-red cell blood group activity from an anti-HLA serum was easily obtained by adsorption on the corresponding Synsorb without loss of anti-HLA activity.
- Subjects :
- Cytotoxicity, Immunologic
Immunology
Cell
Human leukocyte antigen
Biochemistry
ABO Blood-Group System
Lewis Blood Group Antigens
HLA Antigens
Isoantibodies
Genetics
medicine
Humans
Immunology and Allergy
Immunosorbent Techniques
biology
Red Cell
Elution
Chemistry
General Medicine
Hydrogen-Ion Concentration
Molecular biology
Red blood cell
medicine.anatomical_structure
biology.protein
Antibody
Subjects
Details
- ISSN :
- 13990039 and 00012815
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Tissue Antigens
- Accession number :
- edsair.doi.dedup.....a3aeb02558ed6c2bc34ec89aa21d9f88
- Full Text :
- https://doi.org/10.1111/j.1399-0039.1985.tb00948.x