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Additional file 1: Figure S1. of Extreme MHC class I diversity in the sedge warbler (Acrocephalus schoenobaenus); selection patterns and allelic divergence suggest that different genes have different functions
- Publication Year :
- 2017
- Publisher :
- Figshare, 2017.
-
Abstract
- Schematic overview of the primer design approach. PaDo primers, marked with red arrows, used for amplification and cloning of cDNA from 4 sedge warblers Acrocephalus schoenobaenus individuals. Hn primers (Westerdahl et al. 2004), designed for great reed warblers A. arundinaceus, used for obtaining whole exon 3 sequences in 4 individuals for both gDNA and cDNA. Subsequently sequences obtained for cDNA were used for designing specific sedge warbler primers (HnallaN and Hn46N) that amplify whole range of exon 3 MHC class I in sedge warblers, excluding a range of pseudogenes. (PDF 1364 kb)
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a3af2496e1c502ebc7ce75c0156b75ec
- Full Text :
- https://doi.org/10.6084/m9.figshare.c.3819346_d1