Additional file 1: Figure S1. of Extreme MHC class I diversity in the sedge warbler (Acrocephalus schoenobaenus); selection patterns and allelic divergence suggest that different genes have different functions

Schematic overview of the primer design approach. PaDo primers, marked with red arrows, used for amplification and cloning of cDNA from 4 sedge warblers Acrocephalus schoenobaenus individuals. Hn primers (Westerdahl et al. 2004), designed for great reed warblers A. arundinaceus, used for obtaining whole exon 3 sequences in 4 individuals for both gDNA and cDNA. Subsequently sequences obtained for cDNA were used for designing specific sedge warbler primers (HnallaN and Hn46N) that amplify whole range of exon 3 MHC class I in sedge warblers, excluding a range of pseudogenes. (PDF 1364 kb)