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Granule Cell Survival is Deficient in PAC1−/− Mutant Cerebellum
- Source :
- Journal of Molecular Neuroscience, Journal of Molecular Neuroscience, Humana Press, 2008, 36 (1-3), pp.38-44. ⟨10.1007/s12031-008-9066-6⟩
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- International audience; PACAP exerts neuroprotective effects during development, especially in the cerebellum where PAC1 receptor and ligand are both expressed. However, while previous studies using PACAP injections in postnatal animals defined trophic effects of exogenous peptide, the role of endogenous PACAP remains unexplored. Here, we used PAC1(-/-) mice to investigate the role of PACAP receptor signaling in postnatal day 7 cerebellum. There was no difference in DNA synthesis in the cerebellar EGL of PAC1(-/-) compared to wild type animals, assessed using thymidine incorporation and BrdU immunohistochemistry. In contrast, we found that a significant proportion of newly generated neurons were eliminated before they successfully differentiated in the granule cell layer. In aggregate, these results suggest that endogenous PACAP plays an important role in cell survival during cerebellar development, through the activation of the PAC1 receptor.
- Subjects :
- endocrine system
medicine.medical_specialty
Cerebellum
Cell Survival
Endogeny
Biology
Mice
Cellular and Molecular Neuroscience
Internal medicine
medicine
Animals
Humans
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Receptor
Mice, Knockout
Neurons
DNA synthesis
Caspase 3
Neurogenesis
Wild type
DNA
General Medicine
Granule cell
Mice, Inbred C57BL
Endocrinology
medicine.anatomical_structure
Apoptosis
Pituitary Adenylate Cyclase-Activating Polypeptide
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
hormones, hormone substitutes, and hormone antagonists
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Subjects
Details
- ISSN :
- 15591166 and 08958696
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Neuroscience
- Accession number :
- edsair.doi.dedup.....a3be1b252a291125cb5c4bafa6702ef6