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Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Public Library of Science, 2017, 11 (12), pp.e0005978. ⟨10.1371/journal.pntd.0005978⟩, PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0005978 (2017), PLOS Neglected Tropical Diseases
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Abstract
- Phlebotomine sandflies are vectors of phleboviruses that cause sandfly fever or meningitis with significant implications for public health. Although several strains of these viruses had been isolated in Iran in the late 1970's, there was no recent data about the present situation at the outset of this study. Entomological investigations performed in 2009 and 2011 in Iran collected 4,770 sandflies from 10 different regions. Based on morphological identification, they were sorted into 315 pools according to species, sex, trapping station and date of capture. A phlebovirus, provisionally named Dashli virus (DASHV), was isolated from one pool of Sergentomyia spp, and subsequently DASHV RNA was detected in a second pool of Phlebotomus papatasi. Genetic and phylogenetic analyses based on complete coding genomic sequences indicated that (i) DASHV is most closely related to the Iranian isolates of Sandfly fever Sicilian virus [SFSV], (ii) there is a common ancestor to DASHV, Sandfly fever Sicilian- (SFS) and SFS-like viruses isolated in Italy, India, Turkey, and Cyprus (lineage I), (iii) DASHV is more distantly related with Corfou and Toros viruses (lineage II) although common ancestry is supported with 100% bootstrap, (iii) lineage I can be subdivided into sublineage Ia including all SFSV, SFCV and SFTV except those isolated in Iran which forms sublineage Ib (DASHV). Accordingly, we suggest to approve Sandfly fever Sicilian virus species consisting of the all aforementioned viruses. Owing that most of these viruses have been identified in human patients with febrile illness, DASHV should be considered as a potential human pathogen in Iran.<br />Author summary Phlebotomine sandflies are vectors of phleboviruses that cause sandfly fever or meningitis with significant implications for public health. Although several strains of these viruses had been isolated in Iran in the late 1970's, there was no recent data about the present situation at the outset of this study. Entomological investigations performed in 2009 and 2011 in Iran collected 4,770 sandflies from 10 different regions. A phlebovirus, provisionally named Dashli virus (DASHV), was isolated / detected in two pools. DASHV strain was isolated in cell culture and complete genome sequence was determined. Sequence analysis indicated that (i) DASHV is most closely related to the Iranian isolates of Sandfly fever Sicilian virus [SFSV], a virus that is known to cause self-resolutive incapacitating febrile illness in humans, (ii) there is a common ancestor to DASHV and all other variants of SFSV isolated in Italy, India, Turkey, and Cyprus (lineage I), (iii) DASHV is more distantly related with Corfou and Toros viruses (lineage II) although common ancestry is supported with 100% bootstrap, (iii) lineage I can be subdivided into sublineage Ia including all SFSV strains, whereas Iranian viruses are most closely related and should be individualized as DASHV (sublineage Ib). Although discovered first in the 1940's, SFSV is still listed as "tentative species" by the International Committee for Taxonomy of Viruses. Based on the results described in this study, we propose to approve Sandfly fever Sicilian virus species. Owing that most of these viruses have been identified in human patients with febrile illness, DASHV should be considered as a potential human pathogen in Iran.
- Subjects :
- 0301 basic medicine
Male
Phlebovirus
RNA viruses
Fevers
Iran
Disease Vectors
Pathology and Laboratory Medicine
Database and Informatics Methods
Phlebotomus Fever
Bunyaviruses
Medicine and Health Sciences
Sandfly Fever Sicilian Virus
Phylogeny
Data Management
Genetics
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
biology
Phylogenetic tree
lcsh:Public aspects of medicine
Phylogenetic Analysis
Gene Pool
3. Good health
Phylogenetics
Infectious Diseases
Medical Microbiology
Viral Pathogens
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Viruses
RNA, Viral
Female
Gene pool
Pathogens
Sequence Analysis
Research Article
Computer and Information Sciences
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
Bioinformatics
Genome, Viral
Research and Analysis Methods
Microbiology
Virus
03 medical and health sciences
Signs and Symptoms
Amino Acid Sequence Analysis
Diagnostic Medicine
Animals
Humans
Evolutionary Systematics
Microbial Pathogens
Taxonomy
Evolutionary Biology
Base Sequence
Population Biology
Sequence Analysis, RNA
Public Health, Environmental and Occupational Health
Organisms
Biology and Life Sciences
lcsh:RA1-1270
biology.organism_classification
Rift Valley fever virus
Sandfly
Insect Vectors
Sand Flies
Species Interactions
030104 developmental biology
Microbial genetics
Psychodidae
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLOS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....a3d10bb0bdb282596c02d0d5e477c0db
- Full Text :
- https://doi.org/10.1371/journal.pntd.0005978