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Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test
- Source :
- Journal of Antimicrobial Chemotherapy, Journal of Antimicrobial Chemotherapy, 2018, 73 (12), pp.3359-3367. ⟨10.1093/jac/dky330⟩, Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2018, 73 (12), pp.3359-3367. ⟨10.1093/jac/dky330⟩, The Journal of antimicrobial chemotherapy, (2018)
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- Background Polymyxins are currently considered a last-resort treatment for infections caused by MDR Gram-negative bacteria. Recently, the emergence of carbapenemase-producing Enterobacteriaceae has accelerated the use of polymyxins in the clinic, resulting in an increase in polymyxin-resistant bacteria. Polymyxin resistance arises through modification of lipid A, such as the addition of phosphoethanolamine (pETN). The underlying mechanisms involve numerous chromosome-encoded genes or, more worryingly, a plasmid-encoded pETN transferase named MCR. Currently, detection of polymyxin resistance is difficult and time consuming. Objectives To develop a rapid diagnostic test that can identify polymyxin resistance and at the same time differentiate between chromosome- and plasmid-encoded resistances. Methods We developed a MALDI-TOF MS-based method, named the MALDIxin test, which allows the detection of polymyxin resistance-related modifications to lipid A (i.e. pETN addition), on intact bacteria, in Results Using a characterized collection of polymyxin-susceptible and -resistant Escherichia coli, we demonstrated that our method is able to identify polymyxin-resistant isolates in 15 min whilst simultaneously discriminating between chromosome- and plasmid-encoded resistance. We validated the MALDIxin test on different media, using fresh and aged colonies and show that it successfully detects all MCR-1 producers in a blindly analysed set of carbapenemase-producing E. coli strains. Conclusions The MALDIxin test is an accurate, rapid, cost-effective and scalable method that represents a major advance in the diagnosis of polymyxin resistance by directly assessing lipid A modifications in intact bacteria.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.drug_class
Polymyxin
030106 microbiology
Drug resistance
Microbial Sensitivity Tests
medicine.disease_cause
Microbiology
Bacterial genetics
Lipid A
03 medical and health sciences
Drug Resistance, Bacterial
medicine
Escherichia coli
Pharmacology (medical)
Polymyxins
Pharmacology
biology
Chemistry
Escherichia coli Proteins
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Plasmid-mediated resistance
Chromosomes, Bacterial
biology.organism_classification
Enterobacteriaceae
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Infectious Diseases
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
lipids (amino acids, peptides, and proteins)
Bacteria
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 03057453 and 14602091
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy, Journal of Antimicrobial Chemotherapy, 2018, 73 (12), pp.3359-3367. ⟨10.1093/jac/dky330⟩, Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2018, 73 (12), pp.3359-3367. ⟨10.1093/jac/dky330⟩, The Journal of antimicrobial chemotherapy, (2018)
- Accession number :
- edsair.doi.dedup.....a3e3d1c2d41bcecc613ec8aa5bd0b9d6