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c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD
- Publication Year :
- 2023
- Publisher :
- Taylor & Francis, 2023.
-
Abstract
- Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of –10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery.
- Subjects :
- Surgical resection
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Nanoparticle
Poloxamer
macromolecular substances
02 engineering and technology
Peptides, Cyclic
03 medical and health sciences
Drug Delivery Systems
0302 clinical medicine
Cell Line, Tumor
Glioma
Quantum Dots
medicine
Animals
Humans
Fluorescent Dyes
Microbubbles
Chemistry
business.industry
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
Fluorescence
Rats
nervous system diseases
Disease Models, Animal
Surgery, Computer-Assisted
Ultrasonic Waves
Quantum dot
030220 oncology & carcinogenesis
Nanoparticles
Administration, Intravenous
0210 nano-technology
Nuclear medicine
business
Biotechnology
Glioblastoma
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a3ea20215c0b5d6447b7e280444cfa0d
- Full Text :
- https://doi.org/10.6084/m9.figshare.23618622