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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae
- Source :
- Mycobiology, Vol 46, Iss 1, Pp 72-78 (2018), Mycobiology
- Publication Year :
- 2018
- Publisher :
- Taylor & Francis Group, 2018.
-
Abstract
- The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Auricularia
Biology
01 natural sciences
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Molecular marker
lcsh:Botany
Botany
Auricularia auricula-judae
Gene
Research Articles
sequence-related amplified polymorphism
Sequence-related amplified polymorphism
food and beverages
Edible fungus
biology.organism_classification
lcsh:QK1-989
sequence characterized amplified region
030104 developmental biology
Infectious Diseases
chemistry
Genetic marker
fruiting body pattern
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20929323 and 12298093
- Volume :
- 46
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Mycobiology
- Accession number :
- edsair.doi.dedup.....a3eee32559a60640e6e0ce309875582f