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Erosive Arthritis and Hepatic Granuloma Formation Induced by Peptidoglycan Polysaccharide in Rats Is Aggravated by Prasugrel Treatment
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 7, p e69093 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- Administration of the thienopyridine P2Y12 receptor antagonist, clopidogrel, increased the erosive arthritis induced by peptidoglycan polysaccharide (PG-PS) in rats or by injection of the arthritogenic K/BxN serum in mice. To determine if the detrimental effects are caused exclusively by clopidogrel, we evaluated prasugrel, a third-generation thienopyridine pro-drug, that contrary to clopidogrel is mostly metabolized into its active metabolite in the intestine. Prasugrel effects were examined on the PG-PS-induced arthritis rat model. Erosive arthritis was induced in Lewis rats followed by treatment with prasugrel for 21 days. Prasugrel treated arthritic animals showed a significant increase in the inflammatory response, compared with untreated arthritic rats, in terms of augmented macroscopic joint diameter associated with significant signs of inflammation, histomorphometric measurements of the hind joints and elevated platelet number. Moreover, fibrosis at the pannus, assessed by immunofluorescence of connective tissue growth factor, was increased in arthritic rats treated with prasugrel. In addition to the arthritic manifestations, hepatomegaly, liver granulomas and giant cell formation were observed after PG-PS induction and even more after prasugrel exposure. Cytokine plasma levels of IL-1 beta, IL-6, MIP1 alpha, MCP1, IL-17 and RANTES were increased in arthritis-induced animals. IL-10 plasma levels were significantly decreased in animals treated with prasugrel. Overall, prasugrel enhances inflammation in joints and liver of this animal model. Since prasugrel metabolites inhibit neutrophil function ex-vivo and the effects of both clopidogrel and prasugrel metabolites on platelets are identical, we conclude that the thienopyridines metabolites might exert non-platelet effects on other immune cells to aggravate inflammation.
- Subjects :
- Prasugrel
lcsh:Medicine
Arthritis
Autoimmunity
030204 cardiovascular system & hematology
Pharmacology
Piperazines
0302 clinical medicine
Platelet
Prodrugs
lcsh:Science
Multidisciplinary
Granuloma
Hematology
Clopidogrel
3. Good health
Liver
Platelet aggregation inhibitor
Cytokines
Medicine
Female
medicine.symptom
medicine.drug
Research Article
Platelets
Drugs and Devices
Ticlopidine
Thienopyridine
Immunology
Inflammation
Rheumatoid Arthritis
Peptidoglycan
Thiophenes
Cardiovascular Pharmacology
03 medical and health sciences
Adverse Reactions
Rheumatology
medicine
Animals
Biology
030203 arthritis & rheumatology
business.industry
lcsh:R
Immunity
medicine.disease
Arthritis, Experimental
Rats
Rats, Inbred Lew
Immune System
lcsh:Q
Joints
business
Prasugrel Hydrochloride
Platelet Aggregation Inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a3f9cbafe067ca478e78aa033e5a0a4a