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Redox regulation of T-cell turnover by the p13 protein of human T-cell leukemia virus type 1: distinct effects in primary versus transformed cells
- Source :
- Blood. 116(1)
- Publication Year :
- 2010
-
Abstract
- The present study investigated the function of p13, a mitochondrial protein of human T-cell leukemia virus type 1 (HTLV-1). Although necessary for viral propagation in vivo, the mechanism of function of p13 is incompletely understood. Drawing from studies in isolated mitochondria, we analyzed the effects of p13 on mitochondrial reactive oxygen species (ROS) in transformed and primary T cells. In transformed cells (Jurkat, HeLa), p13 did not affect ROS unless the cells were subjected to glucose deprivation, which led to a p13-dependent increase in ROS and cell death. Using RNA interference we confirmed that expression of p13 also influences glucose starvation-induced cell death in the context of HTLV-1–infected cells. ROS measurements showed an increasing gradient from resting to mitogen-activated primary T cells to transformed T cells (Jurkat). Expression of p13 in primary T cells resulted in their activation, an effect that was abrogated by ROS scavengers. These findings suggest that p13 may have a distinct impact on cell turnover depending on the inherent ROS levels; in the context of the HTLV-1 propagation strategy, p13 could increase the pool of “normal” infected cells while culling cells acquiring a transformed phenotype, thus favoring lifelong persistence of the virus in the host.
- Subjects :
- Gene Expression Regulation, Viral
Programmed cell death
T-Lymphocytes
Immunology
Genetic Vectors
Green Fluorescent Proteins
Retroviridae Proteins
Mitochondrion
medicine.disease_cause
Biochemistry
Jurkat cells
Cell Line
HeLa
Jurkat Cells
Transduction, Genetic
medicine
Humans
Cells, Cultured
Human T-lymphotropic virus 1
Microscopy, Confocal
biology
Reverse Transcriptase Polymerase Chain Reaction
Lentivirus
Cell Biology
Hematology
T lymphocyte
biology.organism_classification
medicine.disease
Cell biology
Mitochondria
Leukemia
Host-Pathogen Interactions
RNA Interference
Carcinogenesis
Reactive Oxygen Species
Oxidation-Reduction
HeLa Cells
Subjects
Details
- ISSN :
- 15280020
- Volume :
- 116
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....a417b9a56f754b9a3b541d1a74497342