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Effect of N-methionine-free, bacterially synthesized recombinant human granulocyte-macrophage colony-stimulating Factor in a primate model

Authors :
Shibuya T
Takashi Okamura
Yoshiyuki Niho
S Taniguchi
Takanori Teshima
Koichi Akashi
Mamoru Harada
Source :
European Journal of Haematology. 44:100-105
Publication Year :
2009
Publisher :
Wiley, 2009.

Abstract

We demonstrate the in vivo effects of bacterially synthesized, N-methionine-free recombinant human granulocyte-macrophage colony stimulating factor (rh GM-CSF) using a crab-eating monkey model. Monkeys were treated with cyclophosphamide (60 mg/kg) and administered with rh GM-CSF (30 micrograms/kg/d) subcutaneously (s.c.) for 7 days. Within 12 h, a transient increase of neutrophils (greater than 15.0 x 10(9)/l) was observed, and complete recovery of WBC counts was obtained by d 9 (d 16 in control monkeys). Neutrophils and eosinophils were absolutely increased (greater than 8 x 10(9)/l) on d 10. Readministration of rh GM-CSF (30 micrograms/kg/d, s.c.) for 3 d (including control monkeys) revealed absolute increases of neutrophils, eosinophils, monocytes and platelets. A two-fold increase of granulocyte/macrophage colony-forming units was also seen in the bone marrow, while the number of burst-forming units-erythroid was not affected. These data indicate that rh GM-CSF of this type stimulates granulopoiesis and thrombopoiesis in vivo.

Details

ISSN :
16000609 and 09024441
Volume :
44
Database :
OpenAIRE
Journal :
European Journal of Haematology
Accession number :
edsair.doi.dedup.....a42c158c24e340125857a899885669a2
Full Text :
https://doi.org/10.1111/j.1600-0609.1990.tb00358.x