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Effect of N-methionine-free, bacterially synthesized recombinant human granulocyte-macrophage colony-stimulating Factor in a primate model
- Source :
- European Journal of Haematology. 44:100-105
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- We demonstrate the in vivo effects of bacterially synthesized, N-methionine-free recombinant human granulocyte-macrophage colony stimulating factor (rh GM-CSF) using a crab-eating monkey model. Monkeys were treated with cyclophosphamide (60 mg/kg) and administered with rh GM-CSF (30 micrograms/kg/d) subcutaneously (s.c.) for 7 days. Within 12 h, a transient increase of neutrophils (greater than 15.0 x 10(9)/l) was observed, and complete recovery of WBC counts was obtained by d 9 (d 16 in control monkeys). Neutrophils and eosinophils were absolutely increased (greater than 8 x 10(9)/l) on d 10. Readministration of rh GM-CSF (30 micrograms/kg/d, s.c.) for 3 d (including control monkeys) revealed absolute increases of neutrophils, eosinophils, monocytes and platelets. A two-fold increase of granulocyte/macrophage colony-forming units was also seen in the bone marrow, while the number of burst-forming units-erythroid was not affected. These data indicate that rh GM-CSF of this type stimulates granulopoiesis and thrombopoiesis in vivo.
- Subjects :
- Male
medicine.medical_specialty
Granulocyte
Biology
Granulopoiesis
Colony-Forming Units Assay
Leukocyte Count
Methionine
Colony-Stimulating Factors
Reference Values
In vivo
Internal medicine
Escherichia coli
medicine
Animals
Platelet
Thrombopoiesis
Growth Substances
Cyclophosphamide
Platelet Count
Granulocyte-Macrophage Colony-Stimulating Factor
Hematology
General Medicine
Hematopoietic Stem Cells
Colony-stimulating factor
Recombinant Proteins
Macaca fascicularis
medicine.anatomical_structure
Granulocyte macrophage colony-stimulating factor
Endocrinology
Mutation
Bone marrow
medicine.drug
Subjects
Details
- ISSN :
- 16000609 and 09024441
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- European Journal of Haematology
- Accession number :
- edsair.doi.dedup.....a42c158c24e340125857a899885669a2
- Full Text :
- https://doi.org/10.1111/j.1600-0609.1990.tb00358.x