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Serologic dissection of HLA-D specificities by the use of monoclonal antibodies
- Source :
- Immunogenetics. 17:485-495
- Publication Year :
- 1983
- Publisher :
- Springer Science and Business Media LLC, 1983.
-
Abstract
- To study the gene products of the HLA complex, we produced two monoclonal antibodies, termed HU-18 and HU-23. They were active in complement-dependent cytotoxicity and detected B-cell alloantigens encoded by a locus (or loci) linked to HLA. When three types of HLA-DR4 homozygous B-cell lines with different HLA-D specificities were tested for reactivity with HU-18 and HU-23, they displayed distinct reaction patterns depending on the HLA-D specificities they possessed: EBV-Wa (HLA-DYT homozygous), negative for both HU-18 and HU-23; KT2 and KOB (HLA-DKT2 homozygous), positive only for HU-18; and ER (HLA-Dw4 homozygous), positive for both. These differential reaction patterns were further confirmed by testing against a panel of 17 HLA-DR4-positive peripheral blood lymphocytes with known HLA-D specificities. Thus, these monoclonal antibodies allow us to identify HLA-DYT, HLA-DKT2, and HLA-Dw4 solely by serologic methods. This is the first clearcut serologic identification of these three HLA-DR4-associated HLA-D specificities, which have been indistinguishable by conventional serology and identified only by cellular techniques. It is hoped that immunochemical investigations using HU-18 and HU-23 will advance our understanding of the HLA-D region on a molecular level.
- Subjects :
- Genetic Linkage
medicine.drug_class
Genes, MHC Class II
Immunology
Histocompatibility Antigens Class II
Antibodies, Monoclonal
Locus (genetics)
Human leukocyte antigen
Biology
Monoclonal antibody
Virology
Molecular biology
Human genetics
Serology
Gene product
Epitopes
Antibody Specificity
Peripheral blood lymphocyte
Genetics
medicine
Humans
Lymphocytes
Gene
Cells, Cultured
Subjects
Details
- ISSN :
- 14321211 and 00937711
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Immunogenetics
- Accession number :
- edsair.doi.dedup.....a434285af2e7c1dcf378f902de56aaac