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Human Platelet Vesicles Exhibit Distinct Size and Proteome
- Source :
- Journal of Proteome Research. 16:2333-2338
- Publication Year :
- 2017
- Publisher :
- American Chemical Society (ACS), 2017.
-
Abstract
- In the past 50 years, isolated blood platelets have had restricted use in wound healing, cancer therapy, and organ and tissue transplant, to name a few. The major obstacle for its unrestricted use has been, among others, the presence of ultrahigh concentrations of growth factors and the presence of both pro-angiogenic and anti-angiogenic proteins. To overcome this problem requires the isolation and separation of the membrane bound secretory vesicles containing the different factors. In the current study, high-resolution imaging of isolated secretory vesicles from human platelets using atomic force microscopy (AFM) and mass spectrometry enabled characterization of the remaining vesicles size and composition following their immunoseparation. The remaining vesicles obtained following osmotic lysis, when subjected to immunoseparation employing antibody to different vesicle-associated membrane proteins (VAMPs), demonstrate for the first time that VAMP-3-, VAMP-7-, and VAMP-8-specific vesicles each possesses distinct size range and composition. These results provide a window into our understanding of the heterogeneous population of vesicles in human platelets and their stability following both physical manipulation using AFM and osmotic lysis of the platelet. This study further provides a platform for isolation and the detailed characterization of platelet granules, with promise for their future use in therapy. Additionally, results from the study demonstrate that secretory vesicles of different size found in cells reflect their unique and specialized composition and function.
- Subjects :
- Blood Platelets
0301 basic medicine
Proteome
Vesicle-Associated Membrane Protein 3
Chemical Fractionation
Biology
Microscopy, Atomic Force
Tandem mass spectrometry
Biochemistry
Article
R-SNARE Proteins
03 medical and health sciences
0302 clinical medicine
Osmotic Pressure
Humans
Immunoprecipitation
Platelet
Cells, Cultured
030222 orthopedics
Secretory Vesicles
Vesicle
Molecular Sequence Annotation
General Chemistry
Secretory Vesicle
Cell biology
Cytolysis
030104 developmental biology
Membrane protein
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Wound healing
Subjects
Details
- ISSN :
- 15353907 and 15353893
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Journal of Proteome Research
- Accession number :
- edsair.doi.dedup.....a468e27e452ca6d6d7239b32a7cecd5c