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Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries
- Source :
- International Journal of Molecular Sciences, Vol 17, Iss 3, p 324 (2016), International Journal of Molecular Sciences; Volume 17; Issue 3; Pages: 324, International Journal of Molecular Sciences
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Accumulating evidence demonstrates that microRNA-29 (miR-29) expression is prominently decreased in patients with hepatic fibrosis, which consequently stimulates hepatic stellate cells’ (HSCs) activation. We used a cDNA microarray study to gain a more comprehensive understanding of genome-wide gene expressions by adjusting miR-29a expression in a bile duct-ligation (BDL) animal model. Methods: Using miR-29a transgenic mice and wild-type littermates and applying the BDL mouse model, we characterized the function of miR-29a with regard to cholestatic liver fibrosis. Pathway enrichment analysis and/or specific validation were performed for differentially expressed genes found within the comparisons. Results: Analysis of the microarray data identified a number of differentially expressed genes due to the miR-29a transgene, BDL, or both. Additional pathway enrichment analysis revealed that TGF-β signaling had a significantly differential activated pathway depending on the occurrence of miR-29a overexpression or the lack thereof. Furthermore, overexpression was found to elicit changes in Wnt/β-catenin after BDL. Conclusion: This study verified that an elevated miR-29a level could alleviate liver fibrosis caused by cholestasis. Furthermore, the protective effects of miR-29a correlate with the downregulation of TGF-β and associated with Wnt/β-catenin signal pathway following BDL.
- Subjects :
- Liver Cirrhosis
Male
0301 basic medicine
Genetically modified mouse
Microarray
bile duct ligation
Transgene
Biology
Article
Catalysis
Wnt signaling pathway
lcsh:Chemistry
Inorganic Chemistry
Transcriptome
Mice
03 medical and health sciences
miR-29a
cholestasis
liver fibrosis
TGF-β signaling pathway
Transforming Growth Factor beta
Animals
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Microarray analysis techniques
Organic Chemistry
General Medicine
Up-Regulation
Computer Science Applications
MicroRNAs
030104 developmental biology
Liver
lcsh:Biology (General)
lcsh:QD1-999
Immunology
Cancer research
Hepatic stellate cell
Intercellular Signaling Peptides and Proteins
Hepatic fibrosis
Signal Transduction
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a487ba442b39605c18077710637e21bf