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Accumulation of Eu3+ chelates in cells expressing or not P-glycoprotein: Implications for blood-brain barrier crossing

Authors :
Carlos F. G. C. Geraldes
Gabriel Gras
Nacira Darghal
Nadia Bouchemal
Arlette Garnier-Suillerot
Milena Salerno
Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT)
Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Source :
Journal of Inorganic Biochemistry, Journal of Inorganic Biochemistry, Elsevier, 2010, 104 (1), pp.47-54. ⟨10.1016/j.jinorgbio.2009.09.026⟩
Publication Year :
2010
Publisher :
HAL CCSD, 2010.

Abstract

1 - Article; Alzheimer's disease (AD) is the most commonly form of dementia in the elderly. The development of molecules able to detect biomarkers characteristic of AD is critical to its understanding and treatment. However, such molecules must be able to pass blood-brain barrier (BBB) which is a major impediment to the entry of many therapeutic drugs into the brain. Such a limitation applies to the development of magnetic resonance imaging molecular neuroimaging agents using biomarkers of AD-like P-amyloid deposits, as the common extracellular contrast agents (CAs) are not able to cross an intact BBB. In this work, we have studied the ability of a series of simple Eu3+ Complexes to enter cells overexpressing or not the ABCB1 (P-gp or P-glycoprotein) protein, which is expressed at the BBB and in human embryonic astrocytes. The intracellular uptake of the Eu3+ complexes of linear and macrocyclic polyaminocarboxylate ligands with different charges and lipophilicities was followed by atomic absorption spectrometry. Based on biochemical argument, we propose that lipophilic contrast agents can be efficiently taken up by cells and accumulate inside mitochondria when they are positively charged. The important point is that they are not P-gp substrates. which is one of the major obstacles for them to cross the BBB.

Details

Language :
English
ISSN :
01620134
Database :
OpenAIRE
Journal :
Journal of Inorganic Biochemistry, Journal of Inorganic Biochemistry, Elsevier, 2010, 104 (1), pp.47-54. ⟨10.1016/j.jinorgbio.2009.09.026⟩
Accession number :
edsair.doi.dedup.....a48a08143996598bd9597e44c113851c
Full Text :
https://doi.org/10.1016/j.jinorgbio.2009.09.026⟩