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Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations
- Source :
- Molecules, Vol 17, Iss 11, Pp 12925-12937 (2012), Molecules; Volume 17; Issue 11; Pages: 12925-12937, Molecules
- Publication Year :
- 2012
- Publisher :
- MDPI AG, 2012.
-
Abstract
- A high performance liquid chromatographic (HPLC) method with diode array detection (DAD) was established for simultaneous determination of seven main bioactive components in San-ao decoction and its series of formulae (San-ao decoction, Wu-ao decoction, Qi-ao decoction and Jia-wei San-ao decoction). Seven compounds were analyzed simultaneously with a XTerra C18 column (4.6 mm × 250 mm, 5 µm) using a linear gradient elution of a mobile phase containing acetonitrile (A) and a buffer solution (0.02 mol/L potassium dihydrogen phosphate and adjusted to pH 3 using phosphoric acid) (B); the flow rate was 1.0 mL/min. The sample was detected with DAD at 210, 254 and 360 nm and the column was maintained at 30 °C. All the compounds showed good linearity (r2 > 0.9984) in the tested concentration range. The precisions were evaluated by intra-day and inter-day tests, and relative standard deviation (R.S.D.) values within the range of 0.83%–2.53% and 0.64%–2.77% were reported, respectively. The recoveries of the quantified compounds were observed to cover a range from 95.34% and 104.82% with R.S.D. values less than 2.72%. The validated method was successfully applied for the simultaneous determination of seven main bioactive components including ephedrine (1), amygdalin (2), liquiritin (3), benzoic acid (4), isoliquiritin (5), formononetin (6) and glycyrrhizic acid (7) in San-ao decoction and its series of formulae. The results also showed a wide variation in the content of the identified active compounds in these samples, which could also be helpful to illustrate the drug interactions after some herbs combined in different formulations.
- Subjects :
- San-ao decoction
formulations
Amygdalin
Pharmaceutical Science
Decoction
Armeniacae Semen Amarum
Signal-To-Noise Ratio
HPLC-DAD
High-performance liquid chromatography
Article
Analytical Chemistry
lcsh:QD241-441
chemistry.chemical_compound
Chalcone
Glucosides
lcsh:Organic chemistry
Limit of Detection
Drug Discovery
Medicine, Chinese Traditional
Physical and Theoretical Chemistry
Phosphoric acid
Chromatography, High Pressure Liquid
Benzoic acid
Ephedrine
Chromatography
Elution
Glycyrrhizae Radix et Rhizoma
Organic Chemistry
Buffer solution
Benzoic Acid
Reference Standards
Glycyrrhizic Acid
Isoflavones
chemistry
Ephedrae Herba
Chemistry (miscellaneous)
Calibration
Flavanones
Molecular Medicine
Spectrophotometry, Ultraviolet
Liquiritin
Drugs, Chinese Herbal
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 17
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....a48a2dc945b7ef2558f0d88d2bbb0036