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Transcription of mouse mammary tumor virus: identification of a candidate mRNA for the long terminal repeat gene product
- Source :
- Journal of Virology. 46:42-49
- Publication Year :
- 1983
- Publisher :
- American Society for Microbiology, 1983.
-
Abstract
- We have examined an assortment of preneoplastic and neoplastic mouse mammary tissues for the presence of an mRNA which could encode the putative long terminal repeat gene product of mouse mammary tumor virus. We report here the detection of a novel mouse mammary tumor virus-specific, polyadenylic acid-containing transcript in certain preneoplastic and neoplastic mammary tissue of BALB/c mice. The molecule is 1.6 kilobases in length and contains sequences from the transcriptional leader and the U3 region of the proviral DNA. The upstream terminus of the 3' information lies 75 to 80 nucleotides from the beginning of the long terminal repeat open reading frame, in close proximity to a consensus splice acceptor in the DNA. The transcript was detected in hormonally or chemically induced neoplastic, preneoplastic, and lactating mammary tissue of BALB/c mice, but not in preneoplastic or tumor tissue induced by exogenous viruses in any strain of mice examined. This implies that the RNA we observed is transcribed from an endogenous provirus template.
- Subjects :
- Genes, Viral
Transcription, Genetic
RNA Splicing
Immunology
Mice, Inbred Strains
Microbiology
Gene product
Mice
Transcription (biology)
Virology
Animals
Tissue Distribution
RNA, Messenger
Repetitive Sequences, Nucleic Acid
Mammary tumor
Messenger RNA
biology
Mouse mammary tumor virus
Mammary Neoplasms, Experimental
Nucleic Acid Hybridization
RNA
Provirus
biology.organism_classification
Molecular biology
Long terminal repeat
Mammary Tumor Virus, Mouse
Insect Science
DNA, Viral
RNA, Viral
Female
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....a48c418ac68f29c477b9fda5ef5c3565