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Monoclonal Antibodies Against Peptidorhamnomannans of Scedosporium apiospermum Enhance the Pathogenicity of the Fungus
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e853 (2010)
- Publication Year :
- 2010
- Publisher :
- Public Library of Science, 2010.
-
Abstract
- Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.<br />Author Summary The incidence of fungal infections has increased dramatically over the last 50 years, largely because of the increasing size of the population at risk, which especially includes immunocompromised hosts. Scedosporium apiospermum is a filamentous fungus that causes a variety of infections, ranging from localized disease to life-threatening disseminated infections. Glycoproteins are molecules present in the fungal surface and are comprised of carbohydrate and protein components. They are involved in different important functions in the fungal cell. Monoclonal antibodies can be used as therapeutic agents for infectious disease, but some factors involved in their efficacy are often not well understood. We found that monoclonal antibodies to glycoproteins present in fungal surface can be nonprotective and can even enhance the disease. The administration of these antibodies can affect functions of the fungal cell and the immune cells, resulting in a survival advantage for the fungus during interactions with the host.
- Subjects :
- lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
medicine.drug_class
viruses
Virulence
Monoclonal antibody
Epitope
Microbiology
Cell Line
03 medical and health sciences
Epitopes
Mice
Antigen
Phagocytosis
medicine
Sporothrix schenckii
Animals
Antibody-dependent enhancement
Scedosporium
Antibodies, Fungal
030304 developmental biology
Glycoproteins
0303 health sciences
Mice, Inbred BALB C
biology
030306 microbiology
lcsh:Public aspects of medicine
Macrophages
Public Health, Environmental and Occupational Health
Antibodies, Monoclonal
lcsh:RA1-1270
Scedosporium apiospermum
biology.organism_classification
Virology
Antibody-Dependent Enhancement
Survival Analysis
Glycopeptide
3. Good health
Microbiology/Immunity to Infections
Disease Models, Animal
Infectious Diseases
Mycoses
Female
Microbiology/Cellular Microbiology and Pathogenesis
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 4
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....a48ef3c213540e2d93fd3406ff06553f