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Protection against influenza A virus by vaccination with a recombinant fusion protein linking influenza M2e to human serum albumin (HSA)
- Source :
- Journal of virological methods. 228
- Publication Year :
- 2014
-
Abstract
- The highly conserved extracellular domain of M2 protein (M2e) of influenza A viruses has limited immunogenicity on its own. Hence, aiming to enhance immunogenicity of M2e protein, optimal approaches remain to be established. In this study, we created recombinant fusion protein vaccines by linking M2e consensus sequence of influenza A viruses with C-terminal domain of human serum albumin (HSA). Then HSA/M2e recombinant fusion protein was studied. Our results showed that HSA/M2e could induce strong anti-M2e specific humoral immune responses in the established mice model. Administration of HSA/M2e with Freund's adjuvant resulted in a higher number of IFN-γ-producing cells compared to HSA/M2e or M2e peptide emulsified in Freund's adjuvant. Furthermore, HSA/M2e was able to reduce viral load in the mice lungs and provide significant protection against lethal challenge with an H1N1 or an H3N2 virus compared to controls. In conclusion, this study has demonstrated a potential vaccine that could provide protection in preventing the threat of influenza outbreak because of rapid variation of the influenza virus.
- Subjects :
- 0301 basic medicine
Cross Protection
Recombinant Fusion Proteins
Freund's Adjuvant
Serum albumin
Biology
medicine.disease_cause
Antibodies, Viral
Virus
Disease Outbreaks
Viral Matrix Proteins
03 medical and health sciences
Interferon-gamma
Mice
0302 clinical medicine
Immune system
Influenza A Virus, H1N1 Subtype
Orthomyxoviridae Infections
Virology
Influenza, Human
Influenza A virus
medicine
Animals
Humans
030212 general & internal medicine
Serum Albumin
Mice, Inbred BALB C
Vaccines, Synthetic
Viral matrix protein
Influenza A Virus, H5N1 Subtype
Immunogenicity
Influenza A Virus, H3N2 Subtype
Vaccination
Viral Load
Fusion protein
Immunity, Humoral
body regions
030104 developmental biology
Freund's adjuvant
Influenza Vaccines
embryonic structures
biology.protein
Female
Subjects
Details
- ISSN :
- 18790984
- Volume :
- 228
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....a4a3c02d549fc96206b98ad69245099f