Back to Search Start Over

Germline ERCC excision repair 6 like 2 ( <scp> ERCC6L2 </scp> ) mutations lead to impaired erythropoiesis and reshaping of the bone marrow microenvironment

Authors :
Hannah Armes
Findlay Bewicke‐Copley
Ana Rio‐Machin
Doriana Di Bella
Céline Philippe
Anna Wozniak
Hemanth Tummala
Jun Wang
Teresa Ezponda
Felipe Prosper
Inderjeet Dokal
Tom Vulliamy
Outi Kilpivaara
Ulla Wartiovaara‐Kautto
Jude Fitzgibbon
Kevin Rouault‐Pierre
Department of Medical and Clinical Genetics
ATG - Applied Tumor Genomics
Research Programs Unit
University of Helsinki
HUSLAB
Medicum
HUS Comprehensive Cancer Center
Clinicum
Helsinki University Hospital Area
Hematologian yksikkö
Source :
British Journal of Haematology. 199:754-764
Publication Year :
2022
Publisher :
Wiley, 2022.

Abstract

Despite the inclusion of inherited myeloid malignancies as a separate entity in the World Health Organization Classification, many established predisposing loci continue to lack functional characterization. While germline mutations in the DNA repair factor ERCC excision repair 6 like 2 (ERCC6L2) give rise to bone marrow failure and acute myeloid leukaemia, their consequences on normal haematopoiesis remain unclear. To functionally characterise the dual impact of germline ERCC6L2 loss on human primary haematopoietic stem/progenitor cells (HSPCs) and mesenchymal stromal cells (MSCs), we challenged ERCC6L2-silenced and patient-derived cells ex vivo. Here, we show for the first time that ERCC6L2-deficiency in HSPCs significantly impedes their clonogenic potential and leads to delayed erythroid differentiation. This observation was confirmed by CIBERSORTx RNA-sequencing deconvolution performed on ERCC6L2-silenced erythroid-committed cells, which demonstrated higher proportions of polychromatic erythroblasts and reduced orthochromatic erythroblasts versus controls. In parallel, we demonstrate that the consequences of ERCC6L2-deficiency are not limited to HSPCs, as we observe a striking phenotype in patient-derived and ERCC6L2-silenced MSCs, which exhibit enhanced osteogenesis and suppressed adipogenesis. Altogether, our study introduces a valuable surrogate model to study the impact of inherited myeloid mutations and highlights the importance of accounting for the influence of germline mutations in HSPCs and their microenvironment.

Details

ISSN :
13652141 and 00071048
Volume :
199
Database :
OpenAIRE
Journal :
British Journal of Haematology
Accession number :
edsair.doi.dedup.....a4b6bd468b25bf4765090e98dc92cb01