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Phlebotomus sergenti (Parrot, 1917) identified as Leishmania killicki host in Ghardaïa, south Algeria
- Source :
- Microbes and Infection, Microbes and Infection, Elsevier, 2011, 13 (7), pp.691-6. ⟨10.1016/j.micinf.2011.02.008⟩
- Publication Year :
- 2010
-
Abstract
- International audience; Since 2005, an outbreak of human cutaneous leishmaniasis (CL) in Ghardaïa, south Algeria, was studied and one output of these investigations was the identification of two Leishmania species, Leishmania major and Leishmania killicki, as the CL causative agents. In the present study, we were curious to focus on sand fly fauna present in this area and detection of Leishmania-positive sand fly females. Sand flies (3717) were collected during two seasons using sticky papers and CDC light traps in urban, rural and sylvatic sites. Twelve Phlebotomus species were identified. Phlebotomus papatasi was dominant in the urban site while Phlebotomus sergenti and Phlebotomus riouxi/chabaudi were dominant in the sylvatic site. Out of 74 P. sergenti females captured by CDC light traps in the sylvatic site populated by Ghardaïas' Gundi (Massoutiera mzabi), three ones were hosting Leishmania promastigotes. PCR-RFLP and sequencing of seven single-copy coding DNA sequences identified the promastigotes as L. killicki. Furthermore, laboratory experiments revealed that L. killicki isolate sampled from a CL patient inhabiting the studied region develop well in P. sergenti females. Our findings strongly suggest that the human cutaneous leishmaniases caused by L. killicki is a zoonotic disease with P. sergenti sand flies acting as hosts and vectors and gundi rodents as reservoirs.
- Subjects :
- Male
MESH: Sequence Analysis, DNA
Veterinary medicine
Leishmania tropica
Urban Population
MESH: Rodentia
030308 mycology & parasitology
Disease Outbreaks
MESH: Phlebotomus
0302 clinical medicine
MESH: Animals
Leishmania major
Phlebotomus
MESH: Disease Outbreaks
Leishmania
0303 health sciences
biology
Gundi
MESH: Urban Population
Infectious Diseases
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Seasons
MESH: Algeria
Polymorphism, Restriction Fragment Length
MESH: Leishmania
030231 tropical medicine
Immunology
MESH: DNA, Protozoan
Leishmaniasis, Cutaneous
Rodentia
MESH: Insect Vectors
MESH: Host-Parasite Interactions
Microbiology
Host-Parasite Interactions
03 medical and health sciences
Cutaneous leishmaniasis
parasitic diseases
medicine
Animals
Humans
Psychodidae
Massoutiera mzabi
MESH: Humans
MESH: Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
DNA, Protozoan
MESH: Leishmaniasis, Cutaneous
biology.organism_classification
medicine.disease
MESH: Male
Insect Vectors
Algeria
MESH: Seasons
MESH: Female
Subjects
Details
- ISSN :
- 1769714X and 12864579
- Volume :
- 13
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Microbes and infection
- Accession number :
- edsair.doi.dedup.....a4bc00ea1ae7806d7bcffa527e58f8d3