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SNAP-Tag-Based Subcellular Protein Labeling and Fluorescent Imaging with Naphthalimides
- Source :
- Chembiochem : a European journal of chemical biology. 18(17)
- Publication Year :
- 2017
-
Abstract
- Genetically encoded technologies provide methods for specific labeling and imaging of proteins, which is essential for the understanding of their subcellular localization and function. Herein, we employ naphthalimide, an efficient two-photon fluorophore, to develop O6-benzylguanine (BG) derivatives for specific labeling of subcellular proteins and fluorescent imaging through the SNAP-tag. The three naphthalimide-BG derivatives, TNI-BG, QNI-BG and ONI-BG are conveniently synthesized through modular "click chemistry" with high yields. All of them show high labeling efficiency with SNAP-tag in solution (1~2 ×103 s-1 M-1) and in bacteria. Among them, ONI-BG shows high specificity to diffused, histone H2B and mitochondria COX8A targeted SNAP-tag in mammalian cells. The protein labeled naphthalimides exhibit high two-photon absorption cross section, which indicates the potential application in protein specific two-photon fluorescent imaging, such as two-photon fluorescent lifetime imaging and two-photon multi-color imaging. Therefore, ONI-BG is a versatile tool for tracking of subcellular proteins via multiple fluorescent techniques.
- Subjects :
- Fluorophore
Recombinant Fusion Proteins
Biology
010402 general chemistry
01 natural sciences
Biochemistry
Electron Transport Complex IV
Histones
chemistry.chemical_compound
Chlorocebus aethiops
Histone H2B
Animals
Humans
Molecular Biology
DNA Modification Methylases
Fluorescent Dyes
010405 organic chemistry
Tumor Suppressor Proteins
Organic Chemistry
Subcellular localization
Fluorescence
0104 chemical sciences
SNAP-tag
Naphthalimides
DNA Repair Enzymes
Microscopy, Fluorescence, Multiphoton
chemistry
COS Cells
Click chemistry
Molecular Medicine
Click Chemistry
Function (biology)
Subjects
Details
- ISSN :
- 14397633
- Volume :
- 18
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Chembiochem : a European journal of chemical biology
- Accession number :
- edsair.doi.dedup.....a4c24bf5bc7d92d6be962c4d8b897575