Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Authors :: Cassandra E. Faux
Ian M. Mackay
Michael D. Nissen
Katherine E. Arden
Terry Nolan
Stephen B. Lambert
Theo P. Sloots
Anne B. Chang
Source :: Emerging Infectious Diseases, Vol 17, Iss 2, Pp 296-298 (2011), Emerging Infectious Diseases
Publication Year :: 2011
Publisher :: Centers for Disease Control and Prevention (CDC), 2011.
Abstract: We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

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