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Phenazine carboxylic acid and its derivative induce osteoblast differentiation in preosteoblastic MC3T3-E1 cells but adipocyte differentiation in pluripotent mesenchymal C3H10T1/2 cells
- Source :
- The Journal of Antibiotics. 70:1146-1149
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Osteoblast and adipocyte are differentiated from mesenchymal stem cells and dysregulation of the differentiation might result in disease, such as osteoporosis and diabetes. To find small compounds that induce osteoblast differentiation, we screened an in-house natural compounds library with mouse preosteoblastic MC3T3-E1 cells using alkaline phosphatase (ALP) expression as an early osteoblast marker. We found that phenazine-1-carboxylic acid (PCA), one of the major phenazine derivatives produced by Pseudomonas, induced osteoblast differentiation in the cells at micromolar concentrations. PCA acted synergistically with an agonist of hedgehog signaling in inducing ALP activity in the cells. We also found that 2-hydroxy-PCA (2H-PCA) induced osteoblast differentiation in the cells but 2-methoxy-PCA and 1-hydroxy-phenazine did not. Unexpectedly, treatment of mouse pluripotent mesenchymal C3H10T1/2 cells with PCA or 2H-PCA induced an obvious morphological change. Oil Red O staining and real-time reverse-transcription PCR analysis revealed that PCA induced not osteoblast differentiation but adipocyte differentiation in C3H10T1/2 cells. These compounds could allow us to investigate the mechanism of osteoblast and adipocyte differentiation in the two model cell systems through a chemical biology approach.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Cell
Chemical biology
Biology
Cell Line
Mice
03 medical and health sciences
chemistry.chemical_compound
Osteogenesis
Pseudomonas
Adipocyte
Drug Discovery
Adipocytes
medicine
Animals
Oil Red O
Pharmacology
Adipogenesis
Osteoblasts
Mesenchymal stem cell
Mesenchymal Stem Cells
Osteoblast
3T3 Cells
Alkaline Phosphatase
Hedgehog signaling pathway
Cell biology
030104 developmental biology
medicine.anatomical_structure
Biochemistry
chemistry
Phenazines
Alkaline phosphatase
Subjects
Details
- ISSN :
- 18811469 and 00218820
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- The Journal of Antibiotics
- Accession number :
- edsair.doi.dedup.....a4da88a2cbf5f47d291392898df9e11b