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Phenazine carboxylic acid and its derivative induce osteoblast differentiation in preosteoblastic MC3T3-E1 cells but adipocyte differentiation in pluripotent mesenchymal C3H10T1/2 cells

Authors :
Yasuko Kohda
Hayamitsu Adachi
Manabu Kawada
Takumi Watanabe
Yoshio Nishimura
Shuichi Sakamoto
Ryuichi Sawa
Masatomi Iijima
Maiko Okada
Source :
The Journal of Antibiotics. 70:1146-1149
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

Osteoblast and adipocyte are differentiated from mesenchymal stem cells and dysregulation of the differentiation might result in disease, such as osteoporosis and diabetes. To find small compounds that induce osteoblast differentiation, we screened an in-house natural compounds library with mouse preosteoblastic MC3T3-E1 cells using alkaline phosphatase (ALP) expression as an early osteoblast marker. We found that phenazine-1-carboxylic acid (PCA), one of the major phenazine derivatives produced by Pseudomonas, induced osteoblast differentiation in the cells at micromolar concentrations. PCA acted synergistically with an agonist of hedgehog signaling in inducing ALP activity in the cells. We also found that 2-hydroxy-PCA (2H-PCA) induced osteoblast differentiation in the cells but 2-methoxy-PCA and 1-hydroxy-phenazine did not. Unexpectedly, treatment of mouse pluripotent mesenchymal C3H10T1/2 cells with PCA or 2H-PCA induced an obvious morphological change. Oil Red O staining and real-time reverse-transcription PCR analysis revealed that PCA induced not osteoblast differentiation but adipocyte differentiation in C3H10T1/2 cells. These compounds could allow us to investigate the mechanism of osteoblast and adipocyte differentiation in the two model cell systems through a chemical biology approach.

Details

ISSN :
18811469 and 00218820
Volume :
70
Database :
OpenAIRE
Journal :
The Journal of Antibiotics
Accession number :
edsair.doi.dedup.....a4da88a2cbf5f47d291392898df9e11b